ULB/14 August 2009

From 2009.igem.org

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<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li>
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<li><a class="mainlink" href="/Team:ULB-Brussels/Modeling">Modeling</a></li>
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<li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li>
<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li>
<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li>
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li>
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{| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b"  align="right"
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!align="right"|{{#calendar: title=ULB |year=2009 | month=07}}
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|-
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!align="right"|{{#calendar: title=ULB |year=2009 | month=08}}
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!align="right"|{{#calendar: title=ULB |year=2009 | month=09}}
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|}

Latest revision as of 10:43, 21 October 2009

iGEM Team:ULB-Brussels Wiki


July
MTWTFSS
    [http://2009.igem.org/ULB/1_July_2009 1] [http://2009.igem.org/ULB/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=ULB/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=ULB/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=ULB/6_July_2009&action=edit 6] [http://2009.igem.org/ULB/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_July_2009&action=edit 8] [http://2009.igem.org/ULB/9_July_2009 9] [http://2009.igem.org/wiki/index.php?title=ULB/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=ULB/11_July_2009&action=edit 11] [http://2009.igem.org/ULB/12_July_2009 12]
[http://2009.igem.org/wiki/index.php?title=ULB/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=ULB/14_July_2009&action=edit 14] [http://2009.igem.org/ULB/15_July_2009 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_July_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_July_2009&action=edit 19]
[http://2009.igem.org/ULB/20_July_2009 20] [http://2009.igem.org/ULB/21_July_2009 21] [http://2009.igem.org/ULB/22_July_2009 22] [http://2009.igem.org/ULB/23_July_2009 23] [http://2009.igem.org/ULB/24_July_2009 24] [http://2009.igem.org/ULB/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=ULB/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=ULB/28_July_2009&action=edit 28] [http://2009.igem.org/ULB/29_July_2009 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=ULB/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=ULB/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_August_2009&action=edit 2]
[http://2009.igem.org/ULB/3_August_2009 3] [http://2009.igem.org/ULB/4_August_2009 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_August_2009&action=edit 5] [http://2009.igem.org/ULB/6_August_2009 6] [http://2009.igem.org/ULB/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=ULB/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=ULB/10_August_2009&action=edit 10] [http://2009.igem.org/ULB/11_August_2009 11] [http://2009.igem.org/ULB/12_August_2009 12] [http://2009.igem.org/ULB/13_August_2009 13] [http://2009.igem.org/ULB/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_August_2009&action=edit 16]
[http://2009.igem.org/ULB/17_August_2009 17] [http://2009.igem.org/ULB/18_August_2009 18] [http://2009.igem.org/ULB/19_August_2009 19] [http://2009.igem.org/ULB/20_August_2009 20] [http://2009.igem.org/ULB/21_August_2009 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=ULB/24_August_2009&action=edit 24] [http://2009.igem.org/ULB/25_August_2009 25] [http://2009.igem.org/ULB/26_August_2009 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_August_2009&action=edit 27] [http://2009.igem.org/ULB/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_August_2009&action=edit 30]
[http://2009.igem.org/ULB/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/ULB/1_September_2009 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2] [http://2009.igem.org/ULB/3_September_2009 3] [http://2009.igem.org/ULB/4_September_2009 4] [http://2009.igem.org/ULB/5_September_2009 5] [http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6]
[http://2009.igem.org/ULB/7_September_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8] [http://2009.igem.org/ULB/9_September_2009 9] [http://2009.igem.org/ULB/10_September_2009 10] [http://2009.igem.org/ULB/11_September_2009 11] [http://2009.igem.org/ULB/12_September_2009 12] [http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13]
[http://2009.igem.org/ULB/14_September_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]


A glycerol is ready for bacteria having integrated the RBS.

Plasmids containing the RBS have been purified using the purification site (2x 1500µl of culture). 7µl of each purification were digested with 1µl of XbaI, PstI and 1µl of tampon H.

An electrophoresis on gel confirmed the presence of the plasmids in each tube. Parasites on the first tube are probably due to an incomplete digestion.

14 8.jpg

The quantity of purified plasmids was evaluated using a photometry analysis on a 100x dilution.

All plasmids show a concentration close to 50ng and a very acceptable purity.

hfsG and hfsH have been re-suspended in water (50µl) and then diluted 20 times.

The ligation of the promoter and the RBS in the destination plasmid have been stopped by freezing those at 80°C during 20 min.

Bacteria have been transformed with hfsG, hfsH (to keep a stock of plasmids) and our 2 ligations (in order to check wether it did work or not).

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