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ULB/22 July 2009
From 2009.igem.org
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<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | ||
| - | <li><a class="mainlink" href="/Team:ULB-Brussels/ | + | <li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li> |
<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | ||
Latest revision as of 21:57, 20 October 2009
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Amplification
PCR on:
- hfsG gene
- hfsH gene
- promotor
- terminator
- RBS + RFP
PCR cycles
3 different PCR cycles have been carried out :
- at 55°C
- at 60°C
- a special « deletion programme» (for longer primers)
| Temperature (°C) | DMSO | DMSO | No DMSO | No DMSO |
|---|---|---|---|---|
| 55 | ADN | Control | ADN | Control |
| 60 | ADN | Control | ADN | Control |
| deletion | ADN | Control | ADN | Control |
Running on a agarose gel (55° and 60° programs)
Results
- biobricks : ok
- hfsG and hfsH gene: same results as previously
