ULB/29 July 2009

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(New page: == July 29, 2009 == PCR (programme délétion) de RBS+Promoteur (avec et sans DMSO) en utilisant l’amorce forward du promoteur et l’amorce reverse du RBS<br/> PCR des gènes hfsH et hf...)
 
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== July 29, 2009 ==
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PCR (programme délétion) de RBS+Promoteur (avec et sans DMSO) en utilisant l’amorce forward du promoteur et l’amorce reverse du RBS<br/>
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PCR des gènes hfsH et hfsG avec les nouvelles amorces<br/>  
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<head>
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Migration sur gel du plasmide purifié. Résultat : bande d’intérêt mince<br/>
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*PCR (deletion program) by RBS + promoter (with and without DMSO) using the forward primer of promoter and reverse primer of RBS.
 +
 
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*PCR of '''hFSH''' and '''hfsG''' with new primers.
 +
 
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*Gel migration of purified plasmid.
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*Result: band of interest is thin.

Latest revision as of 10:47, 21 October 2009

iGEM Team:ULB-Brussels Wiki


July
MTWTFSS
    [http://2009.igem.org/ULB/1_July_2009 1] [http://2009.igem.org/ULB/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=ULB/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=ULB/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=ULB/6_July_2009&action=edit 6] [http://2009.igem.org/ULB/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_July_2009&action=edit 8] [http://2009.igem.org/ULB/9_July_2009 9] [http://2009.igem.org/wiki/index.php?title=ULB/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=ULB/11_July_2009&action=edit 11] [http://2009.igem.org/ULB/12_July_2009 12]
[http://2009.igem.org/wiki/index.php?title=ULB/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=ULB/14_July_2009&action=edit 14] [http://2009.igem.org/ULB/15_July_2009 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_July_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_July_2009&action=edit 19]
[http://2009.igem.org/ULB/20_July_2009 20] [http://2009.igem.org/ULB/21_July_2009 21] [http://2009.igem.org/ULB/22_July_2009 22] [http://2009.igem.org/ULB/23_July_2009 23] [http://2009.igem.org/ULB/24_July_2009 24] [http://2009.igem.org/ULB/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=ULB/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=ULB/28_July_2009&action=edit 28] [http://2009.igem.org/ULB/29_July_2009 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=ULB/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=ULB/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_August_2009&action=edit 2]
[http://2009.igem.org/ULB/3_August_2009 3] [http://2009.igem.org/ULB/4_August_2009 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_August_2009&action=edit 5] [http://2009.igem.org/ULB/6_August_2009 6] [http://2009.igem.org/ULB/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=ULB/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=ULB/10_August_2009&action=edit 10] [http://2009.igem.org/ULB/11_August_2009 11] [http://2009.igem.org/ULB/12_August_2009 12] [http://2009.igem.org/ULB/13_August_2009 13] [http://2009.igem.org/ULB/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_August_2009&action=edit 16]
[http://2009.igem.org/ULB/17_August_2009 17] [http://2009.igem.org/ULB/18_August_2009 18] [http://2009.igem.org/ULB/19_August_2009 19] [http://2009.igem.org/ULB/20_August_2009 20] [http://2009.igem.org/ULB/21_August_2009 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=ULB/24_August_2009&action=edit 24] [http://2009.igem.org/ULB/25_August_2009 25] [http://2009.igem.org/ULB/26_August_2009 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_August_2009&action=edit 27] [http://2009.igem.org/ULB/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_August_2009&action=edit 30]
[http://2009.igem.org/ULB/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/ULB/1_September_2009 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2] [http://2009.igem.org/ULB/3_September_2009 3] [http://2009.igem.org/ULB/4_September_2009 4] [http://2009.igem.org/ULB/5_September_2009 5] [http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6]
[http://2009.igem.org/ULB/7_September_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8] [http://2009.igem.org/ULB/9_September_2009 9] [http://2009.igem.org/ULB/10_September_2009 10] [http://2009.igem.org/ULB/11_September_2009 11] [http://2009.igem.org/ULB/12_September_2009 12] [http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13]
[http://2009.igem.org/ULB/14_September_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]


  • PCR (deletion program) by RBS + promoter (with and without DMSO) using the forward primer of promoter and reverse primer of RBS.
  • PCR of hFSH and hfsG with new primers.
  • Gel migration of purified plasmid.
  • Result: band of interest is thin.