ULB/7 September 2009

From 2009.igem.org

iGEM Team:ULB-Brussels Wiki


July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30

Results of ligation:

  • Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
  • Phenotypic tests:
    • Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
    • Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.

Extracting of plasmides 9, 10, 5, 6 and 2.

Transformation of 5 ligations carried out on 9/5.

Dubbing of the ligation 1 with IPTG in IPTG medium.

  • Results:No fluorescence

Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.

Following ligations:

  1. LIG1: Tube 4 (insert) + Tube 5
  2. Lig2: Tube 5 (insert) + Tube 9