ULB/7 September 2009

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iGEM Team:ULB-Brussels Wiki


July
MTWTFSS
    [http://2009.igem.org/ULB/1_July_2009 1] [http://2009.igem.org/ULB/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=ULB/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=ULB/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=ULB/6_July_2009&action=edit 6] [http://2009.igem.org/ULB/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_July_2009&action=edit 8] [http://2009.igem.org/ULB/9_July_2009 9] [http://2009.igem.org/wiki/index.php?title=ULB/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=ULB/11_July_2009&action=edit 11] [http://2009.igem.org/ULB/12_July_2009 12]
[http://2009.igem.org/wiki/index.php?title=ULB/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=ULB/14_July_2009&action=edit 14] [http://2009.igem.org/ULB/15_July_2009 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_July_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_July_2009&action=edit 19]
[http://2009.igem.org/ULB/20_July_2009 20] [http://2009.igem.org/ULB/21_July_2009 21] [http://2009.igem.org/ULB/22_July_2009 22] [http://2009.igem.org/ULB/23_July_2009 23] [http://2009.igem.org/ULB/24_July_2009 24] [http://2009.igem.org/ULB/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=ULB/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=ULB/28_July_2009&action=edit 28] [http://2009.igem.org/ULB/29_July_2009 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=ULB/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=ULB/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_August_2009&action=edit 2]
[http://2009.igem.org/ULB/3_August_2009 3] [http://2009.igem.org/ULB/4_August_2009 4] [http://2009.igem.org/wiki/index.php?title=ULB/5_August_2009&action=edit 5] [http://2009.igem.org/ULB/6_August_2009 6] [http://2009.igem.org/ULB/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=ULB/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=ULB/10_August_2009&action=edit 10] [http://2009.igem.org/ULB/11_August_2009 11] [http://2009.igem.org/ULB/12_August_2009 12] [http://2009.igem.org/ULB/13_August_2009 13] [http://2009.igem.org/ULB/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_August_2009&action=edit 16]
[http://2009.igem.org/ULB/17_August_2009 17] [http://2009.igem.org/ULB/18_August_2009 18] [http://2009.igem.org/ULB/19_August_2009 19] [http://2009.igem.org/ULB/20_August_2009 20] [http://2009.igem.org/ULB/21_August_2009 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=ULB/24_August_2009&action=edit 24] [http://2009.igem.org/ULB/25_August_2009 25] [http://2009.igem.org/ULB/26_August_2009 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_August_2009&action=edit 27] [http://2009.igem.org/ULB/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_August_2009&action=edit 30]
[http://2009.igem.org/ULB/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/ULB/1_September_2009 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2] [http://2009.igem.org/ULB/3_September_2009 3] [http://2009.igem.org/ULB/4_September_2009 4] [http://2009.igem.org/ULB/5_September_2009 5] [http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6]
[http://2009.igem.org/ULB/7_September_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8] [http://2009.igem.org/ULB/9_September_2009 9] [http://2009.igem.org/ULB/10_September_2009 10] [http://2009.igem.org/ULB/11_September_2009 11] [http://2009.igem.org/ULB/12_September_2009 12] [http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13]
[http://2009.igem.org/ULB/14_September_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]

Results of ligation:

  • Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
  • Phenotypic tests:
    • Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
    • Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.

Extracting of plasmides 9, 10, 5, 6 and 2.

Transformation of 5 ligations carried out on 9/5.

Dubbing of the ligation 1 with IPTG in IPTG medium.

  • Results:No fluorescence

Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.

Following ligations:

  1. LIG1: Tube 4 (insert) + Tube 5
  2. Lig2: Tube 5 (insert) + Tube 9
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