ULB/9 July 2009
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<html xmlns="http://www.w3.org/1999/xhtml"> | <html xmlns="http://www.w3.org/1999/xhtml"> | ||
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<meta http-equiv="content-type" content="text/html;charset=utf-8" /> | <meta http-equiv="content-type" content="text/html;charset=utf-8" /> | ||
<title>iGEM Team:ULB-Brussels Wiki</title> | <title>iGEM Team:ULB-Brussels Wiki</title> | ||
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- | } | + | <!-- Auto-Reload script for Dev --> |
+ | <script language="javascript" type="text/javascript"> | ||
+ | <!-- | ||
+ | function timedRefresh(timeoutPeriod) { | ||
+ | setTimeout("location.reload(true);",timeoutPeriod); | ||
+ | } | ||
+ | // --> | ||
+ | </script> | ||
+ | <style type="text/css"> | ||
+ | /* Design for https://2009.igem.org/Team:ULB-Brussels | ||
- | + | Université Libre de Bruxelles | |
- | + | */ | |
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- | + | width: 910px; | |
- | + | font-family: Arial; | |
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- | + | } | |
- | + | html body div.header { | |
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- | + | margin-top: -21px; /* strange... */ | |
- | + | } | |
- | + | ||
- | + | /* ===== banner ===== */ | |
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- | + | /* banner = large image on the top of the page, begind the title */ | |
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- | + | html body div.header div.banner { | |
+ | height: 171px; | ||
+ | background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png); | ||
+ | background-repeat: no-repeat; | ||
+ | } | ||
- | + | html body div.header div.banner h1 { | |
- | + | padding-top: 50px; | |
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- | + | text-align: center; | |
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- | + | border: none; | |
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- | + | color: #4E7DB7; | |
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- | + | html body div.header ul { | |
- | + | /* these are common to both 'menu' and 'submenu' */ | |
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- | + | margin-bottom: 0px; | |
- | + | ||
- | + | list-style-type: none; | |
- | + | list-style-image: none; | |
+ | } | ||
- | + | /* ===== menu ===== */ | |
- | + | /* menu = horizontal bar containing the main links to other sections of the wiki */ | |
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- | + | html body div.header div.menu { | |
- | + | background-color: #3E6DA4; | |
- | + | color: white; | |
+ | |||
+ | height: 30px; | ||
+ | padding: 0px; | ||
+ | } | ||
- | + | html body div.header div.menu li { | |
+ | float: left; | ||
+ | text-align: center; | ||
+ | |||
+ | width: 120px; | ||
+ | margin: 0px; | ||
+ | margin-top: 10px; | ||
+ | } | ||
+ | |||
+ | html body div.header div.menu li :hover { | ||
+ | color: #5F7EB4; | ||
+ | background-color: #FFFFFF; | ||
- | + | margin: 0px; | |
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- | + | padding-left: 15px; | |
- | + | padding-right: 15px; | |
- | + | padding-top: 4px; | |
- | + | padding-bottom: 3px; | |
+ | } | ||
- | + | /* class 'selection' refers to the 'selected' menu, in order to change it's color to white */ | |
+ | html body div.header div.menu li a.selection { | ||
+ | color: #5F7EB4; | ||
+ | background-color: #FFFFFF; | ||
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+ | } | ||
- | + | html body div.header div.menu li a { | |
- | + | color: white; | |
- | + | text-decoration: none; | |
- | + | } | |
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- | + | ||
- | + | ||
- | + | /* ===== submenu ===== */ | |
- | + | /* submenu = changing horizontal bar containing the main links to subsections of other sections of the wiki */ | |
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- | + | html body div.header div.submenu { | |
- | + | /*background-color: #404040;*/ | |
- | + | background-color: white; | |
- | + | height: 25px; | |
+ | |||
+ | font-size: small; | ||
+ | |||
+ | border-bottom-style: solid; | ||
+ | border-bottom-width: 1px; | ||
+ | border-bottom-color: #AAAAAA; | ||
+ | } | ||
- | + | html body div.header div.submenu li { | |
+ | float: left; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
- | + | margin: 0px; | |
- | + | margin-top: 4px; | |
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- | + | padding-bottom: 3px; | |
+ | } | ||
- | + | html body div.header div.submenu li a { | |
- | + | color: #5F7EB4; | |
- | + | text-decoration: none; | |
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- | + | ||
- | + | ||
- | + | /* ===== panel ===== */ | |
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- | + | ||
- | + | ||
- | + | /* panel = box containing mascotte, contdown and links to sponsors */ | |
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- | + | html body div.panel { | |
- | + | float: right; | |
+ | border-left-style: solid; | ||
+ | border-left-width: 1px; | ||
+ | border-left-color: #AAAAAA; | ||
+ | |||
+ | padding-left: 10px; | ||
+ | margin-left: 10px; | ||
+ | margin-top: 20px; | ||
+ | |||
+ | width: 250px; | ||
+ | font-size: small; | ||
+ | } | ||
+ | /* appearance of the links */ | ||
+ | html body div.panel a { | ||
+ | text-decoration: none; | ||
+ | font-weight: bold; | ||
+ | color: #3E6DA4; | ||
+ | } | ||
- | + | html body div.panel a:hover { | |
- | + | color: #F5B455; | |
- | + | } | |
- | + | ||
- | + | ||
- | + | ||
+ | /* == countdown == */ | ||
- | + | html body div.panel div.countdown { | |
- | + | /*margin-top: 20px; | |
- | + | margin-bottom: 20px; | |
+ | height: 30px;*/ | ||
+ | margin: 20px; | ||
+ | padding: 20px; | ||
+ | } | ||
- | <div | + | .countdown font { |
- | <div | + | padding: 20px; |
- | <h1> | + | margin: 20px; |
+ | color: #5F7EB4; | ||
+ | } | ||
+ | |||
+ | /* == sponsors == */ | ||
+ | |||
+ | html body div.panel div.sponsor { | ||
+ | margin-bottom: 10px; | ||
+ | min-height: 50px; | ||
+ | } | ||
+ | |||
+ | /* appearance of the sponsor's logos */ | ||
+ | html body div.panel div.sponsor img { | ||
+ | width: 50px; | ||
+ | height: 50px; | ||
+ | border-style: none; | ||
+ | float: left; | ||
+ | margin-right: 7px; | ||
+ | } | ||
+ | |||
+ | /* ===== main ===== */ | ||
+ | |||
+ | html body div.main { | ||
+ | font-size: small; | ||
+ | padding-left: 10px; | ||
+ | padding-right: 10px; | ||
+ | width: 910; | ||
+ | text-align: justify; | ||
+ | } | ||
+ | |||
+ | html body div.main p.abstract { | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | |||
+ | html body div.main div.welcome img { | ||
+ | width: 626px; | ||
+ | height: 150px; | ||
+ | margin-top: 10px; | ||
+ | border-style: none; | ||
+ | } | ||
+ | |||
+ | html body div.footer { | ||
+ | } | ||
+ | |||
+ | </style> | ||
+ | |||
+ | </head> | ||
+ | |||
+ | <!-- *** Uncomment next line to enable autorefresh (developper mode) *** --> | ||
+ | <!-- <body onload="JavaScript:timedRefresh(5000);"> --> | ||
+ | <!-- <body> --> | ||
+ | <body> | ||
+ | <!-- | ||
+ | header : box on the top of the page containing the banner and links to sections and subsections of | ||
+ | the wiki. | ||
+ | --> | ||
+ | <div class="header"> | ||
+ | |||
+ | <div class="banner"> | ||
+ | <h1></h1> | ||
</div> | </div> | ||
- | <div | + | <div class="menu"> |
<ul> | <ul> | ||
- | <li><a class="mainlink" href="/Team:ULB-Brussels | + | <li><a class="mainlink" href="/Team:ULB-Brussels">Home</a></li> |
- | + | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Team">Team</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Team">Team</a></li> | ||
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<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | ||
- | |||
<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | ||
- | + | <li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li> | |
- | <li><a class="mainlink" href="/Team:ULB-Brussels/ | + | <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> |
- | + | ||
- | <li><a class="mainlink" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | + | |
- | + | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
- | <div | + | <div class="submenu"> |
- | < | + | <ul> |
+ | <!-- | ||
<li><a href="#english">English</a></li> | <li><a href="#english">English</a></li> | ||
<li><a href="#french">French</a></li> | <li><a href="#french">French</a></li> | ||
+ | |||
+ | |||
<li><a href="#dutch">Dutch</a></li> | <li><a href="#dutch">Dutch</a></li> | ||
<li><a href="#german">German</a></li> | <li><a href="#german">German</a></li> | ||
<li><a href="#spanish">Spanish</a></li> | <li><a href="#spanish">Spanish</a></li> | ||
<li><a href="#italian">Italiano</a></li> | <li><a href="#italian">Italiano</a></li> | ||
- | </ | + | <li><a href="#chinese">Chinese</a></li> |
+ | --> | ||
+ | |||
+ | |||
+ | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
- | + | <!-- | |
- | < | + | panel : box on the right containing a countdown, links to the sponsors |
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- | + | [ REMOVED ] | |
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- | < | + | <!-- |
- | + | main : main part of the page, containing the text | |
+ | --> | ||
+ | <div class="main"> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="footer"> | ||
+ | </div> | ||
- | + | </body> | |
- | + | </html> | |
- | + | ||
- | |||
+ | <html> | ||
+ | <style type="text/css"> | ||
- | |||
- | + | table.calendar { margin: 0; padding: 10px; } | |
+ | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | ||
+ | table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } | ||
+ | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } | ||
+ | table.month td.today { background-color:#ddd; } | ||
+ | table.month td { | ||
+ | border: none; | ||
+ | margin: 0; | ||
+ | padding: 1pt 1.5pt; | ||
+ | font-weight: bold; | ||
+ | font-size: 12pt; | ||
+ | text-align: right; | ||
+ | background-color: #eee; | ||
+ | } | ||
+ | #bodyContent table.month a { background:none; padding:0 } | ||
+ | .day-active { color:blue } | ||
+ | .day-empty { color:grey } | ||
- | |||
- | |||
- | < | + | </style> |
+ | </html> | ||
- | + | {| align="right" | |
+ | {| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b" align="right" | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=07}} | ||
+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=08}} | ||
+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=09}} | ||
- | + | |} | |
- | + | ||
- | + | ||
- | + | ||
- | + | Choice of bacteria strains : | |
- | + | ||
+ | * ''Caulobacter crescentus'' (strain CB15N) | ||
+ | * ''E. Coli'' (strain K12 or DG1) | ||
+ | |||
+ | === Constructions === | ||
+ | |||
+ | '''Construction 1''' | ||
+ | |||
+ | As ''Caulobacter crescentus'' genes of interest have'' E. coli'' homologs, we’ll first transfer '''HfsG''' et '''HfsH''' genes through the [http://www.partsregistry.org/Assembly_standard_21 assembly standard 21] and we hope ''E. Coli'' will be able to produce the adhesive material. | ||
+ | |||
+ | Forward and reverse primers are designed. | ||
+ | |||
+ | hfsG and hfsH share a single nucleotide HfsG : ---TG'''A''' ; HfsH : '''A'''TG--- | ||
+ | ==> a blunt-end ligation(?) is possible. | ||
+ | |||
+ | Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
Latest revision as of 21:54, 20 October 2009
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Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1)
Constructions
Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standard 21 and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG--- ==> a blunt-end ligation(?) is possible.
Terminator chosen : BBa_B0015