User:DavidC/19 August 2009

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=== Wednesday the 19th ===
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<i>Mycobacterium avium</i> transplanting on Lowensteim medium (two tubes). <br>
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First polymerase chain reaction (PCR) on the lambda phage D protein. <br>
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Cycle: <br>
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Hot-Start : 94°C, 2 minutes. <br>
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DNA denaturation : 94°C, 1 minute. <br>
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Primers annealing : 59°C, 1 minute. <br>
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Primers extension : 72°C, 1 minute. <br>
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D protein electrophoresis. <br>
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No results. <br>

Revision as of 01:25, 21 October 2009

framless



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Wednesday the 19th

Mycobacterium avium transplanting on Lowensteim medium (two tubes).

First polymerase chain reaction (PCR) on the lambda phage D protein.

Cycle:

Hot-Start : 94°C, 2 minutes.
DNA denaturation : 94°C, 1 minute.
Primers annealing : 59°C, 1 minute.
Primers extension : 72°C, 1 minute.

D protein electrophoresis.

No results.