User:DavidC/19 August 2009
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+ | === Wednesday the 19th === | ||
+ | |||
+ | <i>Mycobacterium avium</i> transplanting on Lowensteim medium (two tubes). <br> | ||
+ | |||
+ | First polymerase chain reaction (PCR) on the lambda phage D protein. <br> | ||
+ | |||
+ | Cycle: <br> | ||
+ | |||
+ | Hot-Start : 94°C, 2 minutes. <br> | ||
+ | DNA denaturation : 94°C, 1 minute. <br> | ||
+ | Primers annealing : 59°C, 1 minute. <br> | ||
+ | Primers extension : 72°C, 1 minute. <br> | ||
+ | |||
+ | D protein electrophoresis. <br> | ||
+ | |||
+ | No results. <br> |
Revision as of 01:25, 21 October 2009
Wednesday the 19th
Mycobacterium avium transplanting on Lowensteim medium (two tubes).
First polymerase chain reaction (PCR) on the lambda phage D protein.
Cycle:
Hot-Start : 94°C, 2 minutes.
DNA denaturation : 94°C, 1 minute.
Primers annealing : 59°C, 1 minute.
Primers extension : 72°C, 1 minute.
D protein electrophoresis.
No results.