User:DavidC/22 August 2009
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+ | === Saturday the 22th === | ||
+ | |||
+ | ==== DNA extraction ==== | ||
+ | |||
+ | Lambda phage electrophoresis after Lambda DNA extraction. <br> | ||
+ | |||
+ | === PCR ==== | ||
+ | |||
+ | Sample: D protein. <br> | ||
+ | |||
+ | Cycle : <br> | ||
+ | |||
+ | 94°C 2minutes; <br> | ||
+ | (94°C 1 minute; <br> | ||
+ | 50°C 1 minutes; <br> | ||
+ | 72°C 1 minute(s)) X 5; <br> | ||
+ | (94°C 1 minute; <br> | ||
+ | 60°C 1 minute; <br> | ||
+ | 72°C 1 minute(s)) X 35; <br> | ||
+ | 72°C B + 3 minutes; <br> | ||
+ | |||
+ | ==== DNA electrophoresis ==== | ||
+ | |||
+ | 85 Volt, 15 minutes. <br> | ||
+ | 105 Volt, 40 minutes. <br> | ||
+ | Ladder fermentas 1 Kb. <br> | ||
+ | |||
+ | Samples: D protein (D prt) | ||
+ | |||
+ | |||
+ | [[image:lol.png|center]] |
Revision as of 01:43, 21 October 2009
Contents |
Saturday the 22th
DNA extraction
Lambda phage electrophoresis after Lambda DNA extraction.
PCR =
Sample: D protein.
Cycle :
94°C 2minutes;
(94°C 1 minute;
50°C 1 minutes;
72°C 1 minute(s)) X 5;
(94°C 1 minute;
60°C 1 minute;
72°C 1 minute(s)) X 35;
72°C B + 3 minutes;
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
Samples: D protein (D prt)