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User:DavidC/22 August 2009
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| + | === Saturday the 22th === | ||
| + | |||
| + | ==== DNA extraction ==== | ||
| + | |||
| + | Lambda phage electrophoresis after Lambda DNA extraction. <br> | ||
| + | |||
| + | === PCR ==== | ||
| + | |||
| + | Sample: D protein. <br> | ||
| + | |||
| + | Cycle : <br> | ||
| + | |||
| + | 94°C 2minutes; <br> | ||
| + | (94°C 1 minute; <br> | ||
| + | 50°C 1 minutes; <br> | ||
| + | 72°C 1 minute(s)) X 5; <br> | ||
| + | (94°C 1 minute; <br> | ||
| + | 60°C 1 minute; <br> | ||
| + | 72°C 1 minute(s)) X 35; <br> | ||
| + | 72°C B + 3 minutes; <br> | ||
| + | |||
| + | ==== DNA electrophoresis ==== | ||
| + | |||
| + | 85 Volt, 15 minutes. <br> | ||
| + | 105 Volt, 40 minutes. <br> | ||
| + | Ladder fermentas 1 Kb. <br> | ||
| + | |||
| + | Samples: D protein (D prt) | ||
| + | |||
| + | [[image:SA2208.png|center]] | ||
Latest revision as of 14:03, 21 October 2009
Project
Introduction Current therapies Gene therapies DVS Concept Tissue Vector Cell Vector Therapeutic plasmid Conclusion Implementation Tissue targeting Antitumor action Treatment modeling Conclusion
Ressources
BioBricks Material & Method Bibliography
Ethics & Safety
Contents |
Saturday the 22th
DNA extraction
Lambda phage electrophoresis after Lambda DNA extraction.
PCR =
Sample: D protein.
Cycle :
94°C 2minutes;
(94°C 1 minute;
50°C 1 minutes;
72°C 1 minute(s)) X 5;
(94°C 1 minute;
60°C 1 minute;
72°C 1 minute(s)) X 35;
72°C B + 3 minutes;
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
Samples: D protein (D prt)
