August/11 October 2009

From 2009.igem.org

1.colony check

sample        no. of colony
chiba 2             +
chiba 3             ++
chiba 4             +
 31                   +++
 58                    5
 62                    3 
 73                     0
 76                    >20
 77                     7  
 78                    >30


2. min prep

sample                conc.
 1-23L                   20 ng/uL
 1-23L                   113
  22                        196
  47                        167
  40                        130
  25                        164
  31                        31   
  X4                       265
  X4                       269
  59                       142
  70                       325
  72                       281


3.diagtion

K204079

VectorInsert
1-23L3chiba 14
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O11dH2O11
total20uLtotal20uL

K204080

VectorInsert
1-23L3chiba 54
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O11dH2O11
total20uLtotal20uL

K204081

VectorInsert
1-23L2chiba 65
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O9
total20uLtotal20uL

K204082

VectorInsert
1-23L2chiba 75
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O9
total20uLtotal20uL

K204083

VectorInsert
1-23L3chiba 84
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O11dH2O10
total20uLtotal20uL

K204084

VectorInsert
1-23L2chiba 95
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O9
total20uLtotal20uL


K204067

VectorInsert
1-23L62-16L10
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O10dH2O4
total20uLtotal20uL

K204068

VectorInsert
1-23L101-19B5
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O4dH2O4
total20uLtotal20uL

K204071

VectorInsert
1-8K254
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O10
total20uLtotal20uL


K204075

VectorInsert
1-1K101-8K2
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O4dH2O12
total20uLtotal20uL


37 degree , 2hr
gel cut & purification
ligation

4. min prep

sample                conc.
plac CFP               314 ng/uL
ptet CFP               179
plac mOrange      45
ptet mOrange      170



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