August/12 October 2009

From 2009.igem.org

1. min prep

sample        conc.
 C2               14 ng/uL
 C3               14
 C4               13
 68              182
 68              118
 69              161
 69              119
 74              250
 74              243
 78              105

2.digation

K204072

Vector		Insert
2-8E	6	31	10
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	32	dH₂O	28
total	50uL	total	50uL

K204075

Vector		Insert
1-1K	10	1-8K	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	28	dH₂O	36
total	50uL	total	50uL

K204085

Vector		Insert
40	3	1-15L	10
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	35	dH₂O	28
total	50uL	total	50uL

K204086

Vector		Insert
2-3I	3	69	3
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	28	dH₂O	36
total	50uL	total	50uL

K204087

Vector		Insert
62	2	47	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	32
total	50uL	total	50uL

K204088

Vector		Insert
X3	2	69	3
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	35
total	50uL	total	50uL

K204089

Vector		Insert
1-24E	2	69	4
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	34	dH₂O	34
total	50uL	total	50uL

K204090

Vector		Insert
1-23L	5	chiba	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	33	dH₂O	3
total	50uL	total	50uL

↓
37degree , 3hr
gel cut & purification
ligation O/N

3. min prep

sample      conc,
 58              127 ng/uL
 58              229
 62              79
 76              202
 77              175 
 77              184

4.transformation

sample
79 , 80 , 81 , 82 , 83 , 84 , 68 , 71 , 67 , 73

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