August/28 August 2009

From 2009.igem.org

1. Conducted miniprep followed by nanodrop concentration check on the following: 10-A, 10-B, 10-D, 10-E.
Results of nanodrop check are as follows:

        Conc.    260/280  260/230
10-A     35.2     1.98     1.76

10-B     20.7     1.82     1.59

10-D     37.3     1.90     1.84

10-E     23.7     1.94     1.76


2. Conducted transformation for the following ligated parts: (12), (13), (14), (15), (16), (18), (19), (20), (21).
Also BioBrick part 1-6I was transformed as a positive control.
The plated-out cultures were incubated at 17:30pm.

3. Prepared 42 plates of amphicilin-resistance LA culture medium.

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