August/29 August 2009
From 2009.igem.org
1. Colony check of cultures transformed on 8/28. Results were as follows: Part label or Plate location Approx. number of colonies
(12) 10 (13) 20 (14) 20 (15) 20 (16) over 100 (18) 3 (19) 20 (20) 0 (21) 0 1-6I over 100
Plates (18), (20) and (21) were returned to incubator (37 degrees) to check for colony growth on the following day.
2. (12) to (16), (18), (19), and 1-6I were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow.
3. (12), (15), (16) were streaked on a culture plate together with 1-23L as reference vector. Also, (13), (14), (19) were streaked together with 1-2M as reference vector.
Ligation check (by electrophoresis) should be conducted on these tomorrow.