August/29 August 2009

From 2009.igem.org

1. Colony check of cultures transformed on 8/28. Results were as follows: Part label or Plate location Approx. number of colonies

    (12)                                     10
    (13)                                     20
    (14)                                     20
    (15)                                     20
    (16)                                     over 100
    (18)                                     3
    (19)                                     20
    (20)                                     0
    (21)                                     0
    1-6I                                     over 100

Plates (18), (20) and (21) were returned to incubator (37 degrees) to check for colony growth on the following day.


2. (12) to (16), (18), (19), and 1-6I were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow.


3. (12), (15), (16) were streaked on a culture plate together with 1-23L as reference vector. Also, (13), (14), (19) were streaked together with 1-2M as reference vector.
Ligation check (by electrophoresis) should be conducted on these tomorrow.


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