August/30 August 2009

From 2009.igem.org

1. ligation rapid check

Osaka090830.jpg

UP   : 13-A,B,C  ,  14-A,B,C  , 19-A,B,C  , vector ,ladder
DOWN : 12-A,B,C  ,  15-A,B,C  , 16-A,B,C  , vector , ladder


No.13 (RBS+rhlI),K204013

13-A,C : OK 

No.14 (RBS+LasI),K204014

14 -A,C : OK

No.19 (RBS+RFP(orange?)),K204019

19-A,B : OK


No.12 (RBS+cinR+terminater),K204012

12-B  : OK ?

No.15 (RBS+cinI+terminater), K204015

15-A,B,C : OK?

No.16 (RBS+LuxI+terminater) , K204016

16-A,B,C ok?


2.digestion and ligation

digestion with restriction enzyme

K204020

VectorInsert
p(tetR)([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 1-6I])5YFP([http://partsregistry.org/wiki/index.php?title=Part:BBa_E0430 1-8I])5
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O11dH2O4
total20uLtotal20uL


K204021

VectorInsert
p(tetR)([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 1-6I])5CFP([http://partsregistry.org/wiki/index.php?title=Part:BBa_E0420 1-8K])5
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O11dH2O11
total20uLtotal20uL


3.12-B , 13-A , 14-A , 15-A , 16-A , 19-A were inoculated into growth culture solution.
Miniprep should be conducted on the resulting cell cultures tomorrow.


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