August/8 August 2009
From 2009.igem.org
Today we carried out the following procedures (in rough chronological order):
1. Checked the cell cultures transformed and plated out yesterday (8/7) for colony formation and made an approximate count of the number of colonies.
(plate number)-(location on plate) (no. of colonies)
2-12H none 1-12C none 1-12A none 2-24G none 2-18F none 1-6O ~10 1-10K ~10 1-18L ~10
2. Conducted a 'miniprep' to harvest and check concentration of plasmids from 10 sample. (incubated since yesterday 8/7~) Result of Nanodrop concentration check:
(sample No.) (concentration) [260/280] [260/230]
B0015(1-23L) 24.9 ng/uL 1.77 1.77 K118002(2-16F) 36.0 ng/uL 1.77 1.99 I0462(1-8O) 19.3 ng/uL 1.65 1.59 B0034(1-2M) 18.4 ng/uL 1.64 2.01 I742158(1-19B) 40.9 ng/uL 1.56 0.83 K118003(2-16H) 27.7 ng/uL 1.68 1.38 C0076(1-14D) 9.3 ng/uL 1.66 2.31 C0179(2-8M) 9.9 ng/uL 1.65 1.90 S03878(2-16M) 24.6 ng/uL 1.72 1.24 C0077(1-14F) 2.5 ng/uL 1.11 0.25 failure? I1466(1-23J) failure
3. gel electrophoresis
uL | |
EcoRI | 1 |
SpeI | 1 |
10*Buffer No.2 | 2 |
DNA (EpsE) | 5 |
total | 20 |
↓
37°C,22hr
↓
failure (SYBR Safe : 30min???)