August/8 August 2009

From 2009.igem.org

Today we carried out the following procedures (in rough chronological order):

1. Checked the cell cultures transformed and plated out yesterday (8/7) for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies)


            2-12H                        none
            1-12C                        none
            1-12A                        none
            2-24G                        none
            2-18F                        none
                                               
            1-6O                         ~10
            1-10K                        ~10
            1-18L                        ~10


2. Conducted a 'miniprep' to harvest and check concentration of plasmids from 10 sample. (incubated since yesterday 8/7~) Result of Nanodrop concentration check:

(sample No.) (concentration) [260/280] [260/230]


    B0015(1-23L)           24.9 ng/uL     1.77      1.77
    K118002(2-16F)       36.0 ng/uL     1.77      1.99
    I0462(1-8O)            19.3 ng/uL     1.65      1.59
    B0034(1-2M)            18.4 ng/uL     1.64      2.01
    I742158(1-19B)       40.9 ng/uL     1.56      0.83
    K118003(2-16H)       27.7 ng/uL     1.68      1.38
    C0076(1-14D)            9.3 ng/uL     1.66      2.31
    C0179(2-8M)             9.9 ng/uL     1.65      1.90
    S03878(2-16M)         24.6 ng/uL     1.72      1.24
    C0077(1-14F)            2.5 ng/uL     1.11      0.25   failure?
    I1466(1-23J)            failure


3. gel electrophoresis

uL
EcoRI 1
SpeI 1
10*Buffer No.2 2
DNA (EpsE) 5
total 20



37°C,22hr

failure (SYBR Safe : 30min???)

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