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IBB Pune/2 October 2009
From 2009.igem.org
We pay our respects to Mahatma Gandhi
Transformations:
1 LuxR plate 2 well 40 pSB1A2 2 Negative control 3 Ligation product of PR-TorA-GFP 4 Ligation product of PR-YcdB-GFP
Plasmid Isolations:
1 TorA in pSB1Ak3 2 Constitutive promoter-RFP 3 pSB1Ak3
PCR confirmations with VF2+Vr:
1 lac operator 2 TorA-GFP
3 YcdB-GFP 4 PR
5 pSBAK3 6 TorA
7 LuxI 8 pLuxR
9 fast GFP 10 PR-GFP (AK3)
11 pLuxR-LuxI 12 PR-GFP
13 T7P 14 TorA-GFP-PR
15 YcdB-GFP-PR 16 AK3
All the PCR confirmations were successful!
