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• Team:Heidelberg/Notebook measure/NotebookFC
  ...ted at a different position on the plate as shown in the scheme below. The standard deviations of the replicates are indicated by the error bars.]] ...ted at a different position on the plate as shown in the scheme below. The standard deviations of the replicates are indicated by the error bars.]]
  20 KB (2,861 words) - 23:35, 21 October 2009
• Jamboree/Project Abstract/Team Abstracts
  ...ed in OMVs, using a novel Bioscaffold compatible with the current assembly standard. A new version of the award winning BSim software has been developed to stu ...vel applications of cell surface display within Escherichia coli, the gold standard organism for bacterial engineering. Displaying peptides and proteins on a c
  105 KB (15,465 words) - 15:13, 5 November 2009
• Team:Wash U/Protocol
  * SOC media ...rts and positioning them in a destination plasmid. Specific cut sites for standard restriction enzymes are utilized to simplify the protocol and include EcoR1
  32 KB (5,022 words) - 03:49, 22 October 2009
• Team:UQ-Australia/Project
  ...s were picked from plates, and cultured in 3mL of either LB broth or S.O.C media, supplemented with the appropriate antibiotic. These cultures were incubate ===='''Preparation of Ag43 gene for compatability with BioBrick Standard 21'''====
  39 KB (5,617 words) - 02:52, 22 October 2009
• Team:Minnesota/Notebook
  <h2>Standard Protocols We Used in the Wet Lab</h2> [[Media:Bacterial_Culture.pdf|Bacterial Culture Protocols]]
  9 KB (1,309 words) - 21:31, 23 June 2010
• Team:Utah State/Protocols
  ...wo today, then two tomorrow for streaking). Also ensure that 10 ml liquid media is made up per transformation (also for tomorrow).</li> <li>Centrifuge tube(s) at 15K RPM for 3 minutes. Remove SOC media from the -20C freezer and leave out to thaw.</li>
  36 KB (5,584 words) - 03:25, 21 October 2009
• Team:Heidelberg/Notebook synthetic promoters dna
  * Prepare media * Inoculation of LB-Amp media with colony 1,2,3,7,8 and 9
  48 KB (6,939 words) - 23:25, 21 October 2009
• Team:Johns Hopkins-BAG/A New Standard
  ... a novel standard, The Building Block Method, for both the construction of standard parts and their assembly This Building Block standard can be either:
  3 KB (532 words) - 13:41, 15 October 2009
• Team:Valencia/All Definitions
  ...of engineering principles and biological knowledge to design and construct standard parts, devices and systems, and re-design existing biological systems, for *the design of standard parts that does not exist yet or reconstruct other parts it is engineering
  47 KB (6,943 words) - 03:53, 22 October 2009
• Team:Valencia/Human Practice/Definitions
  ...of engineering principles and biological knowledge to design and construct standard parts, devices and systems, and re-design existing biological systems, for the design of standard parts that does not exist yet or reconstruct other parts it is engineering
  46 KB (6,920 words) - 21:34, 15 October 2009
• Team:Alberta/Project/Modeling
  ...utting edge, streamlined tool for determining essential genes in different media conditions, a tool essential for the genome scale construction projects tha ... either added to our master essential gene list or removed. Additionally, media conditions were altered for the cells environment allowing for predictions
  22 KB (3,594 words) - 02:54, 22 October 2009
• Team:Heidelberg/Notebook MaM
  ==Bacteria Growth Media== ==Cell Culture Media==
  62 KB (8,391 words) - 16:35, 20 November 2009
• Team:Minnesota/Parts Characterization
  ...haustive search of the [http://www.partsregistry.org/Main_Page Registry of Standard Parts], we found five promoters that were included in the 2009 iGEM Kit as ...rts characterization and hope that our work continues to maintain the high standard for characterization of parts. We characterized our 5 promoters included wi
  17 KB (2,746 words) - 18:51, 20 October 2009
• Team:Utah State/Notebook
  ...>Instruction given on tri-parental mating. Discussed selective plates and media for tri-parental mating. </p> ...two today, then two tomorrow for streaking). Also ensure that 10 ml liquid media is made up per transformation (also for tomorrow).</li>
  34 KB (5,337 words) - 04:41, 1 October 2010
• Team:Calgary/Lab/Protocol
  ...e foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> <li>Weigh 35g of LB-Agar powder mix per litre of media desired. One litre makes 40-50 plates</li>
  31 KB (4,983 words) - 21:14, 2 October 2009
• Team:TUDelft/Protocols
  * Grow a 5ml overnight culture of cells in LB media. * In the morning, dilute this culture back into 25-50ml of fresh LB media in a 200ml conical flask. You should aim to dilute the overnight culture by
  11 KB (1,852 words) - 19:32, 21 October 2009
• Team:Heidelberg/Notebook promoters cells
  ** 9 am: change media of SREBP transfection from 10-1 (uninduced -> full medium, inhibiting -> DM ** HeLa for standard
  35 KB (4,989 words) - 00:29, 22 October 2009
• Team:PKU Beijing/Parts/Parts improvement
  ==='''The Reversed Standard'''=== Standard assembly employs four reistriction sites, EcoRI, XbaI, SpeI and PstI. EcoR
  7 KB (1,173 words) - 13:50, 21 October 2009
• Team:Imperial College London/Wetlab/Protocols/Glucose delay
  ...gi Specific Promoter Units (SPUs) by a calculator found on the Registry of Standard Biological Parts]. ====Media====
  6 KB (882 words) - 11:04, 2 October 2009
• Team:TUDelft/Ethics results
  ...nimal genome is groundbreaking and raises a lot of questions, primarily by media machinery. How does the synthetic-biology-research community think about hi * The media refers to Venter's research as "new", but the results show that a majority
  52 KB (8,420 words) - 22:51, 21 October 2009