From 2009.igem.org
All 8 grew
Spun 1.5 mL of overnight culture for 5 minutes in microfuge
#1-4 left 5-8 o/n 37C
Aspirated off all but 100microliters of the supernatant and resuspended the pellet by vortexing
Refrig. o/n
Make 2M NaOH (100mL)-- 8g/100mL = 2M
3m Na Acetate (100mL)--26.4g/100mL = 3M
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