Team:Harvard/Restriction Digest

Restriction Digest

1. Find restriction enzymes of choice
2. Use double digest finder to find conditions
3.Add enzymes, DNA, buffers and amount of water necessary
4.Place digestions in 37 degree for correct amount of time and prepare a diagnostic gel

Team

Projects

• Bacteria-to-Yeast Communication
• Characterization
• PCB Biosynthesis in Yeast
• Split Luciferase
• Yeast Cellular Blackboard
• Yeast Red Light District

Parts

References

Lab Notebook

• Daily
• Protocols
• Recipes

Ethics