Team:NYMU-Taipei/PCR

From 2009.igem.org

Usage:

  1. initial denature time
  2. cycle denature time
  3. cycle primer-stick time
  4. cycle assembly time
  5. assembly time

Extra params:

  • Mix:
    • polName=change polymerase name
    • pol=change polymerase amount
    • ddH20=change water amount
    • fp=forward primer name
    • rp=reverse primer name
  • Protocol:
    • cycles=change amount of cycles
    • a0=change the initial denature temperature
    • a1=change the cycle denature temperature
    • b1=change the cycle primer-stick temperature
    • c1=change the cycle assembly temperature
    • c0=change the last assembly temperature
  • stuff
Mix: 50μl
template 1μl
forward primer 1μl
reverse primer 1μl
dNTP 2μl
10x buffer 5μl
taq 0.25μl
ddH20 39.75 μl
Protocol:
1. 94oC: {{{1}}}
2.
30 cycles
94oC: {{{2}}}
55oC: {{{3}}}
72oC: {{{4}}}
3. 72oC: {{{5}}}