Team:Stanford/Project Homeostasis/sequences

Device 1

RBS – SoxR gene – Terminator – Spacer – SoxS Promoter click here

SoxR gene click here

SoxS Promoter click here

RBS - Blh gene click here

RBS - RALDH II gene - Terminator click here

hIL-6 hlyAs click here

Primer Design Guidelines

• Primer Design
  • Guidelines to Create Primers
  • Designing Mutagenic Primers (see page 10)

Resources

• National Center for Biotechnology Information (NCBI)- Get Your Sequences

Home Page

• Parts Registry of BioBricks- Help with Prefix and Suffix

Prefix and Suffix Help

• IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)

Analyze Your Sequence

• NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)

Analzye both Primers

• ApE- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements

Download here

• Ginkgo BioWorks- creates primers w/ BioBrick additions

Design Primers Here

Archived Documents and Work

• Past Primers and Sequences
  • Finalized Original Primers
  • Original Primers