Template:Team:KULeuven/23 September 2009/VanillinProduction

From 2009.igem.org

  • Miniprep of PAMS&Co
part concentration 260/280 260/230
PAMSCO1 115,6
PAMSCO2 102,2
PAMSCO3 71,0
PAMSCO4 137,1
PAMSCO5 127,2
PAMSCO6 106,6
  • All 6 had the correct size
  • First vanille experiment
    • 1 flask with PEFT cells added 80mg Ferrulic accid
    • 1 flask with EFT cells added 80 mg ferrulic acid
    • Took samples at 0, 0', 20, 40, 60, 90, 120, 180, 240 minutes
    • Centrifuged the samples and transfer the supernatant to a tube