Template:Team:KULeuven/28 September 2009/VanillinProduction

From 2009.igem.org

  • Miniprep of PEFT, PAMSCO electroporation, PAMSCOEFT electroporation
part concentration 260/280 260/230
PEFT1 240,3
PEFT2 169,6
PAMSCO1 184,3
PAMSCO2 184,5
PAMSCOT1 232,5
PAMSCOT2 176,6
PAMSCOT3 293,8
PAMSCOT4 123,1
PAMSCOEFT1 87,4
PAMSCOEFT2 206,9
PAMSCOEFT3 218,4
PAMSCOEFT4 188,2
  • Restriction digest with E/S
  • PAMSCOT has the right length!
  • PAMSCOEFT appears to be a self-closed Terminator vector
  • Transfered an additional 6 colonies from PAMSCOEFT to another plate and liquid cultures