Team:Washington/Notebook
From 2009.igem.org
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[[Team:Washington/Notebook/SOEingPCR|SOEing PCR]] | [[Team:Washington/Notebook/SOEingPCR|SOEing PCR]] | ||
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<br> traditional protein purification (IMAC) techniques <br> | <br> traditional protein purification (IMAC) techniques <br> |
Revision as of 23:33, 16 October 2009
Protocols
Supernatant Protein Purification, 50mL
Supernatant Protein Purification, 2mL
Gene Assembly using the NheI and PstI sites
Gene Assembly using the NheI site
Traditional Protein Purification (IMAC)
traditional protein purification (IMAC) techniques
Project Time Line
- Winter Quarter
- Introduction to iGEM
- Synthetic Biology Seminar
- Plan for project ideas
- Spring Quarter
- Narrow down potential projects
- Choose Project
- Order oligos and start synthesizing genes
- Obtained Funding
- Stock Lab
- June
- Sequence genes
- Preliminary binding assays for biotinylated fluorophore
- Introduction to Fold-it as a tool for protein design
- Test target proteins for solubility and expression
- Start assembly of secretion genes
- July
- Develop and perform assays for testing legacy surface display bio-bricks
- Transfer prtDEF contig from secretion system into low copy plasmid
- Test target proteins for functionality
- August
- Finish assembly of secretion system
- Start cell lines containing various forms of secretion system, make competent
- Transform competent cells containing secretion system with target vector
- Test for secretion of target protein
- Start design of new display construct
- September
- Switch secretion system into new cell line, make cells competent
- Transform with target vector
- Test for secretion
- Start Presentation
- Start t-shirt design
- Insert streptavidin into new display vector
- October
- Fine tune secretion assay, adjust controls
- Finalize characterization of legacy parts
- Practice presentation
- Characterize target bio-bricks
- Prepare for Jamboree