Team:Calgary/20 May 2009

(Difference between revisions)

Revision as of 17:42, 28 July 2009

University of Calgary

NOTEBOOK PAGE INDEX Carol Chinmoyee Emily Fahd Iman Jamie Jeremy Katie Kevin Mandy Patrick Prima Stefan Vicki		JULY 23, 2009 (DATE)

		CAROL Amplification of luxCDABE using Taq polymerase The purpose of this experiment was to amplify luxCDABE with Taq polymerase. Gene specific luxCDABE forward and reverse primers were used. Conditions used were: Denature (94 Degrees Celsius, 3 minutes), 30 cycles (Denature (94 Degrees Celsius, 45 Seconds), Anneal (52 Degrees Celsius, 45 Seconds), Extend (72 Degrees Celsius, 6 minutes)), Final Extension (72 Degrees Celsius, 10 minutes), and hold at 4 Degrees Celsius overnight.

		CHINMOYEE Descriptive Title of What You're Doing WIKI CODING HERE

		EMILY Descriptive Title of What You're Doing WIKI CODING HERE

		FAHD Descriptive Title of What You're Doing WIKI CODING HERE

		IMAN Descriptive Title of What You're Doing WIKI CODING HERE

		JAMIE Descriptive Title of What You're Doing WIKI CODING HERE

		JEREMY Descriptive Title of What You're Doing WIKI CODING HERE

		KATIE Descriptive Title of What You're Doing WIKI CODING HERE

		KEVIN Descriptive of What You're Doing WIKI CODING HERE

		MANDY Descriptive Title of What You're Doing WIKI CODING HERE

		PATRICK Descriptive Title of What You're Doing WIKI CODING HERE

		PRIMA Descriptive Title of What You're Doing WIKI CODING HERE

		STEFAN Descriptive Title of What You're Doing WIKI CODING HERE

		VICKI Descriptive Title of What You're Doing WIKI CODING HERE

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-The purpose of this experiment was to amplify ''luxCDABE'' with Taq polymerase. Gene specific ''LUXCDABE'' forward and reverse primers were used. Conditions used were: Denature (94 Degrees Celsius, 3 minutes), 30 cycles (Denature (94 Degrees Celsius, 45 Seconds), Anneal (52 Degrees Celsius, 45 Seconds), Extend (72 Degrees Celsius, 6 minutes)), Final Extension (72 Degrees Celsius, 10 minutes), and hold at 4 Degrees Celsius overnight.
+The purpose of this experiment was to amplify ''luxCDABE'' with Taq polymerase. Gene specific ''luxCDABE'' forward and reverse primers were used. Conditions used were: Denature (94 Degrees Celsius, 3 minutes), 30 cycles (Denature (94 Degrees Celsius, 45 Seconds), Anneal (52 Degrees Celsius, 45 Seconds), Extend (72 Degrees Celsius, 6 minutes)), Final Extension (72 Degrees Celsius, 10 minutes), and hold at 4 Degrees Celsius overnight.
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