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Team:Imperial College London/Wetlab/Protocols/M1
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(New page: {{Imperial/09/TemplateTop}} =Protein Production (PP)= {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" ! Section ! Assay ! Overview and Aims |- s...) |
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* Measure growth rates at 28 degrees Celsius on minimal growth media so that subsequent testing timings etc. can be streamlined | * Measure growth rates at 28 degrees Celsius on minimal growth media so that subsequent testing timings etc. can be streamlined | ||
* Determine the effect of IPTG toxicity on growth w/o any protein production complications <br> | * Determine the effect of IPTG toxicity on growth w/o any protein production complications <br> | ||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| + | | <b>PP2</b>: Lac characterisation | ||
| + | | [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |Lac characterisation]] | ||
| + | |To investigate how RFP production induced by Lac promoter varies with IPTG <br> | ||
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| <b>PP3</b>: Cellulase | | <b>PP3</b>: Cellulase | ||
Latest revision as of 21:49, 4 October 2009
Protein Production (PP)
| Section | Assay | Overview and Aims |
|---|---|---|
| PP1: IPTG Toxicity | IPTG Toxicity | To investigate the effect of our IPTG inducer on growth of our cultures.
|
| PP2: Lac characterisation | Lac characterisation | To investigate how RFP production induced by Lac promoter varies with IPTG |
| PP3: Cellulase | Cellulase | Aims |
| PP4: PAH | PAH | Aims |
