Team:Imperial College London/Drylab/Autoinduction/Model1

From 2009.igem.org

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==+Model analysis===
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===Model analysis===
<b>Assumptions</b><br>
<b>Assumptions</b><br>
*Michaelis Menten Assumptions [https://2009.igem.org/Team:Imperial_College_London/Drylab/Enzyme (see drug kinetics model) ] have been applied in the kinetics of consumption of enzyme and substrate
*Michaelis Menten Assumptions [https://2009.igem.org/Team:Imperial_College_London/Drylab/Enzyme (see drug kinetics model) ] have been applied in the kinetics of consumption of enzyme and substrate
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**The primary Carbon source: Provides repression of the CRP promoter and delays the start of the encapsulation phase (Module 2)
**The primary Carbon source: Provides repression of the CRP promoter and delays the start of the encapsulation phase (Module 2)
**The secondary Carbon Source: Will power the system once the primary source has been used up. Finding the best secondary carbon source can help us draw a relationship with the output yield of colanic acid.  
**The secondary Carbon Source: Will power the system once the primary source has been used up. Finding the best secondary carbon source can help us draw a relationship with the output yield of colanic acid.  
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction/Analysis/Kompala"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html>&nbsp; about the model assumptions and predictions.
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction/Analysis/Kompala"><img style="vertical-align:bottom;" width=90px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Learnmore.png"></a></html>&nbsp; about the model.
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Revision as of 18:10, 6 October 2009



Contents

Model analysis

Assumptions

  • Michaelis Menten Assumptions (see drug kinetics model) have been applied in the kinetics of consumption of enzyme and substrate
  • Effects of competition and induction can be represented by probabilistic variables (u and v) and are directly dependent on the rate of consumption of any given substrate S_i within the mixture
  • More details about assumptions made for each individual variable are described in the paper.



Predictions

  • In a mixture of substrates, glucose is always used up first by the culture, and then any other secondary source present in the medium.
  • The time length of the primary and secondary exponential phases of growth in the biomass depend on the initial concentration of carbon sources.
  • This will help to predict the switching point between two sources.
  • In our project:
    • The primary Carbon source: Provides repression of the CRP promoter and delays the start of the encapsulation phase (Module 2)
    • The secondary Carbon Source: Will power the system once the primary source has been used up. Finding the best secondary carbon source can help us draw a relationship with the output yield of colanic acid.

  about the model.


The system

Simulation results

Basic conclusions

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