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Team:Imperial College London/Temporal Control
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The timeline shows the sequence of occurrence of these events: | The timeline shows the sequence of occurrence of these events: | ||
[[Image:II09_Timeline.png|600px|centre]] | [[Image:II09_Timeline.png|600px|centre]] | ||
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| + | This timeline shows the sequence of events occuring within the system. <br> | ||
| + | *Starting from the top, we have the different carbon sources responsible for autoinduction of the system. The primary carbon source is glucose, as this is preferentially used by the cell. Also present in the media is a secondary carbon source, which is used by the cell after glucose in the media is depleted. By carefully balancing the initial concentrations of these carbon sources, 'The E.ncapsulator' will begin <b>Module 2</b> only once protein production is at sufficiently high levels. | ||
Revision as of 08:53, 12 October 2009
Temporal Control
In our project there are 3 forms of temporal control that have been implemented.
- Chemical induction: Triggers the production of drug of interest using IPTG.
- Autoinduction: Represses encapsulation when glucose levels are high, and kickstarts it once glucose is used up.
- Thermoinduction: Triggers genome deletion when the temperature is increased.
The timeline shows the sequence of occurrence of these events:
This timeline shows the sequence of events occuring within the system.
- Starting from the top, we have the different carbon sources responsible for autoinduction of the system. The primary carbon source is glucose, as this is preferentially used by the cell. Also present in the media is a secondary carbon source, which is used by the cell after glucose in the media is depleted. By carefully balancing the initial concentrations of these carbon sources, 'The E.ncapsulator' will begin Module 2 only once protein production is at sufficiently high levels.
