Team:UAB-Barcelona/Primersp

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Revision as of 22:49, 21 October 2009

Documento sin título

These were the primers we ordered for the nitrite:

• Pnir: EcoR1-Not-Xba— nirk – Spe

Reverse:

                S
    GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC    Annealing temperature = 62 ºC

Forward:

                 E      N       X
     CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC   Annealing temperature= 60 ºC

• nsrR: X – nsrR – S – N- P

Reverse:

                 X     RBS    Linker
     GTCAACGT TCTAGA TAAGGAGG AATATAA ATGAGACTGACGAATTACAGCGATTAC   Annealing temperature = 76 ºC

Forward:

                 P      N       S
     CAGTAGTA CTGCAG GCGGCCGC ACTAGT GCCAAGCTGACCTACAGTTTATTCC       Annealing temperature = 76 ºC