"
Team:Imperial College London/M2/genes
From 2009.igem.org
JamesField (Talk | contribs) (→YbaY) |
JamesField (Talk | contribs) |
||
| Line 20: | Line 20: | ||
===<b>Colanic Acid Biosynthesis:</b>=== | ===<b>Colanic Acid Biosynthesis:</b>=== | ||
| + | |||
| + | |||
| + | |||
| + | <html> | ||
| + | </p><p class="ecoparagraph"> | ||
| + | <map name="MAP79413"> | ||
| + | <area coords="0,55,32,72" onmouseover="return overlib('RcsAB transcriptional dual regulator activates transcription from associated promoter<br><b>Distance to transcription start site:</b> -105.5 (ranging from -99 to -112)<br> | ||
| + | |||
| + | <b>Evidence:</b> Binding of purified proteins [Wehland00], Site mutation [Wehland00]');" onmouseout="return nd();"> | ||
| + | <area coords="32,55,64,72" onmouseover="return overlib('RcsAB transcriptional dual regulator activates transcription from associated promoter<br><b>Distance to transcription start site:</b> -78.5 (ranging from -72 to -85)<br> | ||
| + | |||
| + | <b>Evidence:</b> Gene expression analysis [Ebel99], Automated inference based on similarity to consensus sequences [Ebel99]');" onmouseout="return nd();"> | ||
| + | <area coords="274,41,338,72" onmouseover="return overlib(' <b>Gene:</b> wzb G7106 b2061 ECK2055<br><b>Location:</b> 2,133,679 <- 2,134,122 <br> <b>Product:</b> tyrosine phosphatase');" onmouseout="return nd();"> | ||
| + | <area coords="745,41,816,72" onmouseover="return overlib(' <b>Gene:</b> wcaB G7103 b2058 ECK2052<br><b>Location:</b> 2,130,091 <- 2,130,579 <br> <b>Product:</b> predicted colanic acid biosynthesis acyl transferase');" onmouseout="return nd();"> | ||
| + | <area coords="631,41,745,72" onmouseover="return overlib(' <b>Gene:</b> wcaA G7104 b2059 ECK2053<br><b>Location:</b> 2,130,582 <- 2,131,421 <br> <b>Product:</b> predicted colanic acid biosynthesis glycosyl transferase');" onmouseout="return nd();"> | ||
| + | <area coords="122,41,274,72" onmouseover="return overlib(' <b>Gene:</b> wza G7107 b2062 ECK2056<br><b>Location:</b> 2,134,128 <- 2,135,267 <br> <b>Product:</b> lipoprotein required for capsular polysaccharide translocation through the outer membrane, subunit of capsular polysaccharide export apparatus');" onmouseout="return nd();"> | ||
| + | <area coords="338,41,619,72" onmouseover="return overlib(' <b>Gene:</b> wzc G7105 b2060 ECK2054<br><b>Location:</b> 2,131,514 <- 2,133,676 <br> <b>Product:</b> tyrosine kinase involved in colanic acid biosynthesis, subunit of capsular polysaccharide export apparatus');" onmouseout="return nd();"> | ||
| + | <area coords="16,0,135,89" onmouseover="return overlib('<b>Operon:</b> wzap (Experim. ev.) <BR><b>Tr.Activators:</b> (RcsAB)');" onmouseout="return nd();"> | ||
| + | <area coords="0,0,818,89" onmouseover="return overlib('<b>Operon</b>: wza-wzb-wzc-wcaAB<br><b>Synonyms:</b> OP00290, cps, cpsB, cpsGB');" onmouseout="return nd();"> | ||
| + | </map> | ||
| + | <img src="https://static.igem.org/mediawiki/2009/8/89/Op1.gif" usemap="#MAP79413" border="0"> | ||
| + | |||
| + | </html> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
=====<b><i>wcaF</i> </b>===== | =====<b><i>wcaF</i> </b>===== | ||
Revision as of 19:18, 11 October 2009
Contents |
RcsB
Background:
RcsB is a transcription factor that forms part of a phosphorelay system. In response to membrane stress, RcsB is phosphorylated into its DNA binding form. In this state, it is able to regulate ~5% of the E.coli genome 4.
The Rcs Phosphorelay System:
In this system, RcsC encodes a membrane bound sensor kinase, while RcsB functions as the response regulator. The protein RcsA is an auxiliary activator protein that heterodimerises with RcsB to induce a mucoid phenotype. Levels of RcsA are controlled by the ATP dependent protease Lon.
RcsB upregulates the following genes:
Colanic Acid Biosynthesis:
wcaF
- Putative colanic acid biosynthesis acetyltransferase 2
gmd
Codes for GDP-mannose 4,6-dehydratase which is a subunit of GDP-mannose 4,6-dehydratase. This enzyme is involved in colanic acid biosynthesis.
wcaD
- Predicted colanic acid polymerase
wza
- This codes for a lipoprotein that is required for colanic acid translocation through the outer membrane. The lipoprotein is a subunit of the capsular polysaccharide export apparatus.
fcl
- Codes for a subunit of GDP-fucose synthase. This enzyme is involved in colanic acid biosynthesis.
General Protective:
ivy (Inhibitor of Vertebrate lysozyme)
- Discovered in 2001 as the first bacterial lysozyme inhibitor. This Type-C lysozyme inhibitor resides in the periplasm.1
MilC (Membrane-bound lysozyme inhibitor of Type C lysozyme)
- This is a lipoprotein that resides in the membrane. 1
OsmC (Osmotically-inducible protein C)
- This protein provides defense against oxidative stress caused by exposure to organic hydroperoxides. 2 3
KatE
- Codes for a subunit of heme d synthase / hydroperoxidase. This bacterial catalase protects against osmotic stress.
Global Regulators:
Sra (stationary-phase-induced ribosome-associated)
Sra is a sub-stoichiometric component of the 30S ribosomal subunit that is more abundant at stationary phase than during log phase growth.
rprA
rprA is a gene encoding a small RNA molecule that is involved in the post-transcriptional control of the alternative sigma factor, ("sigma S").
FtsA
FtsA is an essential cell division protein which colocalizes with FtsZ to the septal ring structure.
FtsZ
- Essential cell division protein.
yfbR
- dCMP phosphohydrolase
Membrane Proteins:
bdm (biofilm-dependent modulation protein)
OsmB
- OsmB encodes an outer membrane lipoprotein.
OsmY
Hyperosmotically inducible periplasmic protein
PhnQ
ycfJ
- Uncharacterized putative inner membrane protein.
ygaC
spy
ymgD
- Putative periplasmic protein.
ypeC
- Unknown function
ymgG
YbaY
Predicted outer membrane lipoprotein
References
1) [http://www.ncbi.nlm.nih.gov/pubmed/19136591 The Rcs two-component system regulates expression of lysozyme inhibitors and is induced by exposure to lysozyme]
2) [http://www.ncbi.nlm.nih.gov/pubmed/19346340 Gene Expression Induced in Escherichia coli O157:H7 upon Exposure to Model Apple Juice]
3) [http://www.ncbi.nlm.nih.gov/pubmed/14627744 Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC.]
4) [http://www.ncbi.nlm.nih.gov/pubmed/16153174?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum The Rcs phosphorelay: a complex signal transduction system.]
