"
Team:UNIPV-Pavia/Project/Results
From 2009.igem.org
|
|
|
Results & Conclusions | |
Contents |
Experimental results
In this section a summary of "Ethanol? Whey not!" project results is reported. For more information you can visit Parts Characterization section.
Ethanol fermentation
Survival of E. coli TOP10 in ethanol
Different concentrations of ethanol have been added to LB and M9 supplemented with glycerol selective media and E. coli TOP10 (bearing BBa_B0030 BioBrick) survival, when grown in a microplate reader (37°C, automatic protocol) and in a 50 ml falcon tube (37°C, 220 rpm), has been tested.
| 
|
| 
|
Of the concentrations we tested, in the microplate reader bacteria can grow at concentrations up to 2.5%w/v (LB) and 1.5%w/v (M9), while in the 50 ml falcon tube they can grow at concentrations up to 3.5%w/v (LB) and 2.5%w/v (M9).
In general, in the falcon tube bacteria grow better than in the microplate (see Measurement section for details) and this is confirmed by these data: it seems that the threshold concentration of ethanol is higher for growth in falcon tube than in the microplate (for example, at 2.5%w/v in M9, bacteria can survive in the falcon tube, but not in the microplate).
Ethanol production
A synthetic ethanol-producing operon () has been built up by our team and has been assembled downstream of several promoters or devices:
