Team:Imperial College London/Biobricks

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II09 BBa.png

Biobricks

Coding Sequences

RcsB

RcsB: Colanic acid global regulator
Part BBa_K200000 : [http://partsregistry.org/Part:BBa_K200000 Link] RcsB is a receiver protein in a two component phosphorelay system, which acts as a positive regulator of a number of genes including capsule genes responsible for colanic acid production. This is via the activation of the ugd/cps operon which is required for capsule synthesis.
Colanic acid is a heteropolymer containing glucose, galactose, fucose, and glucuronic acid.

It is important to note that RcsB is a global regulator found in many Enterobacteria. In Salmonella, up to 38 genes were found to be up-regulated by RcsB including ftsZ which affects the rate of cell division. The majority of upregulated genes are associated with enhancing structural integrety and acid resistance. 22 genes were found to be downregualted by RcsB in the same microarray experiment.


Dam

Dam: DNA Adenine Methylase
Part BBa_K200001 : [http://partsregistry.org/Part:BBa_K200001 Link]
The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.


RcsB: Colanic acid global regulator
Part BBa_K200000 : [http://partsregistry.org/Part:BBa_K200000 Link]

Dam: DNA Adenine Methylase
Part BBa_K200001 : [http://partsregistry.org/Part:BBa_K200001 Link]

ygiV (B3023): Colanic acid global regulator
Part BBa_K200002 : [http://partsregistry.org/Part:BBa_K200002 Link]

WaaL Ligase
Part BBa_K200003 : [http://partsregistry.org/Part:BBa_K200003 Link]

OtsA: Part 1 of 2 for trehalose producing enzymes
Part BBa_K200005 : [http://partsregistry.org/Part:BBa_K200005 Link]

OtsB: Part 2 of 2 coding for trehalose producing enzymes
Part BBa_K200006 : [http://partsregistry.org/Part:BBa_K200006 Link]

Cellulase
Part BBa_K200007 : [http://partsregistry.org/Part:BBa_K200007 Link]

PAH : Phenylalanine Hydroxylase
Part BBa_K200008 : [http://partsregistry.org/Part:BBa_K200008 Link]

Coding Sequence for the Restriction Enzyme DpnII
Part BBa_K200009 : [http://partsregistry.org/Part:BBa_K200009 Link]

Coding sequence for the Restriction Enzyme TaqI
Part BBa_K200010 : [http://partsregistry.org/Part:BBa_K200010 Link]

Temperature Sensitive lamda cI Repressor
Part BBa_K200011 : [http://partsregistry.org/Part:BBa_K200011 Link]

Lambda Promoter (cIts responsive)
Part BBa_K200012 : [http://partsregistry.org/Part:BBa_K200012 Link]

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