Team:Imperial College London/Wetlab/Protocols/Calibration

From 2009.igem.org

Revision as of 15:43, 23 September 2009 by Kun (Talk | contribs)

Calibration protocols (CA)

Section Assay Overview and Aims
CA1:Calibration Curves TOP10 Growth * To produce a calibration curve to aid in the normalising of absorbance values. The relation of absorbance reading to number of cells varies with different cell strains. We are therefore doing one for Top-10.
CA2:External GFP fluorescence Link * To relate how the fluorescence observed can be correlated with number of purified GFP molecules
CA3:Internal GFP fluorescence Link * To relate how various intracellular concentrations of GFP leads to different observed fluorescences. This allows us to relate measured fluorescence from cells to actual GFP number

Mr. Gene   Geneart   Clontech   Giant Microbes