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Team:Imperial College London/Wetlab/Protocols/Cellculture
From 2009.igem.org
CC6: Transformation into Top10 using chemical competence
Protocol
1. Switch on water bath to 42 degrees.
2. Aliquot out LB media and place in water bath (roughly 1 ml for each sample)
3. Chill DNA and 15ml round bottom tubes on ice (1 for each sample)
4. When all this is done, take out cells from -80 degrees freezer and thaw on ice.
5. When cells are thawed, add 30ul of cells to each round bottom tube.
6. Add 3ul of registry DNA or 1ul of midi DNA to tube. Swirl gently.
7. S
