| Section
| Assay
| Overview and Aims
|
| CC1: Miniprep
| Link
| * Harvest smaller amounts of genomic DNA
|
| CC2: Cellulase
| Link
| * Harvest larger amount of genomic DNA
|
| CC3: Cell competence
| Link
| * Making cells more competent to taking up DNA
|
| CC4: Ligation
| Link
| * Ligation of different DNA constructs together<
|
| CC5: Transformation (BL21)
| Link
| * Transformation into BL21 cells using electrical shock <
|
| CC6: Transformation (Top10)
| Transformation into Top10
| * Transformation into chemically competent Top10 cells <
|
| CC7: Make LB plates
| Make LB plates
| * Making plates of LB agar for cell culturing
|
| CC7: Extract DNA from registry
| Extracting DNA
| * Extracting the required DNA from the registry
|
| CC13: Genome Prep for Restriction asay
| Genome Prep
| * Preperation of the genomic DNA for restriction digest assay.
|
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