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Team:Aberdeen Scotland/notebook/quorumsensing
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University of Aberdeen - Pico Plumber
iGEM 2009
Quorum Sensing Notebook
Week 1 - Research and Biobrick Rescues (08/06/09 - 12/06/09)
Day 1 Monday (08/06/09)
- Researching the Registry for Biobricks
- Focus on QS module
- Identified K081008, J23100, C0062, K091156, B0034,B0030, B0015, R0061 and R0062 for possible use in our project.
- Other biobricks identified for other sub-projects include R0011.
Day 2 Tuesday (09/06/09)
- Researching the literature for information on Quorum Sensing.
- Researching for information on LVA tags
Day 3 Wednesday (10/06/09)
- Preparing for biobrick rescue and transformation
- Prepare LB and Agar
- Prepare solutions
- Cacl2 solution,
- 1M MgCl2, 1MMgSO4 and 2M glucose solutions (for SOC medium)
- Inoculate e.coli and grow overnight
Day 4 Thursday (11/06/09)
- Rescue Biobricks (K081008, J37033, B0015, J23100, R0011, R0062, K112808, C0051, C0040, I732100, R0051, K145001 and B0034)
- Prepare competent e.coli, using calcium chloride method
- Transform e.coli with biobricks listed above
- grow overnight on agar/amp plates.
Day 5 Friday (12/06/09)
- Results from yesterday indicate that only I732100 was successfully transformed into e.coli
- Identify possible recipient plasmids for QS module (psB4C5).
- Propose a cloning strategy
- Team meeting
Week 2 (15/06/09 - 19/06/09)
Day 1 Monday (08/06/09)
- Researching the Registry for Biobricks
- Focus on QS module
- Identified K081008, J23100, C0062, K091156, B0034,B0030, B0015, R0061 and R0062 for possible use in our project.
- Other biobricks identified for other sub-projects include R0011.
Day 2 Tuesday (09/06/09)
- Researching the literature for information on Quorum Sensing.
- Researching for information on LVA tags
Day 3 Wednesday (10/06/09)
- Preparing for biobrick rescue and transformation
- Prepare LB and Agar
- Prepare solutions
- Cacl2 solution,
- 1M MgCl2, 1MMgSO4 and 2M glucose solutions (for SOC medium)
- Inoculate e.coli and grow overnight
Day 4 Thursday (11/06/09)
- Rescue Biobricks (K081008, J37033, B0015, J23100, R0011, R0062, K112808, C0051, C0040, I732100, R0051, K145001 and B0034)
- Prepare competent e.coli, using calcium chloride method
- Transform e.coli with biobricks listed above
- grow overnight on agar/amp plates.
Day 5 Friday (12/06/09)
- Results from yesterday indicate that only I732100 was successfully transformed into e.coli
- Identify possible recipient plasmids for QS module (psB4C5).
- Propose a cloning strategy
- Team meeting
Week 3 22/06/09 - 26/06/09
Week 4 29/06/09 - 03/07/09
Week 5 05/07/09 - 10/07/09
Week 6 13/07/09 - 16/07/09
Week 7 20/07/09 - 24/07/09
Week 8 27/07/09 - 31/07/09
Week 9 03/08/09 - 07/08/09
Week 10 10/08/09 - 14/08/09
Week 11 17/08/09 - 21/08/09
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