Team:Aberdeen Scotland/parameters

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University of Aberdeen iGEM 2009

Internal Dynamics Parameters

When modeling any process, it is essential to use parameters which reflect reality, otherwise the model will not give accurate predictions. Thus we spend a considerable time researching, analysing and estimating parameters so that our model will reflect real life behaviour as accuratly as possible.

Parameter Description Value Reference
mRNA Degradation of mRNA 0.003 1
X Degradation of X 0.00000802 2
Y Degradation of Y 0.00000802 4
λ-CI Degradation of lambda CI 0.002888 6
LacI Degradation of LacI 0.001155 8
TetR Degradation of TetR 0.00288811 10
Holin Degradation of Holin 0.0002 guessed; half-life of an hour
Endolysin Degradation of Endolysin 0.0002 guessed; half-life of an hour
Antiholin Degradation of Antiholin 0.0002 guessed; half-life of an hour
LuxI Degradation of LuxI 0.002888 tagged; half-life of 4 min
LuxR Degradation of LuxR 0.0002 0
HSL Degradation of HSL 0.00016667 0; value for AHL
Protein Translation rate of Protein 0.1 2
Rate of production of HSL from LuxI 0.45 2
HSL Rate of diffusion of HSL in/out of the cell 0.4 2
IPTG Rate of diffusion of IPTG in/out of the cell 0.014 2
P Rate of formation of the HSL-LuxI complex 0.00010 2
-P Rate of dissociation of the HSL-LuxI complex 0.003 2
-P Rate of dissociation of the HSL-LuxI complex 0.003 2