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Team:British Columbia
From 2009.igem.org
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| - | + | =<font color="#00CC66">E.coli</font> <font color="#FFCC66">Traffic</font> <font color="#FF0000">Light</font>: <br> A ''flexible'', ''modular'', and ''transparent'' system for multi-level assessment of variable inputs.'= | |
| - | + | Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips. | |
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| - | + | Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light"). | |
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| - | + | Click the colours of the traffic light to learn about its different subparts! | |
| + | <!-- [[Image:BritishColumbia-Trafficlight.png|center|400px]] --> | ||
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| + | <html> | ||
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| + | <img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight"> | ||
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| + | <map name="trafficlight"> | ||
| + | <area shape="circle" | ||
| + | coords="774,121,54" | ||
| + | alt="Sensors: This links to the pBAD promoters that sense the arabinose." | ||
| + | title="Sensors: This links to the pBAD promoters that sense the arabinose." | ||
| + | href="/Team:British_Columbia/pBAD" | ||
| + | /> | ||
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| + | <area shape="circle" | ||
| + | coords="775,236,55" | ||
| + | alt="Lock and Key: this controls when the colors are produced" | ||
| + | title="Lock and Key: this controls when the colors are produced" | ||
| + | href="/Team:British_Columbia/LockandKey" | ||
| + | /> | ||
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| + | <area shape="circle" | ||
| + | coords="774,350,54" | ||
| + | alt="The Jammer: this mRNA sequence blocks the key from opening the lock." | ||
| + | title="The Jammer: this mRNA sequence blocks the key from opening the lock." | ||
| + | href="/Team:British_Columbia/Jammer" | ||
| + | /> | ||
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| + | </map> | ||
| + | </html> | ||
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| + | <!-- | ||
| + | [[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]] | ||
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Latest revision as of 03:58, 22 October 2009
E.coli Traffic Light:
A flexible, modular, and transparent system for multi-level assessment of variable inputs.'
Biosensors have a diverse variety of real-world functions, ranging from measuring blood glucose levels in diabetes patients to assessing environmental contamination of trace toxins. The majority of these sensors are highly specific for a single input, and their outputs often require specialized equipment such as surface plasmon resonance chips.
Our project aims to create a biosensor that recognizes a specific target and alters its output fluorescence from green, to yellow, to red as a function of concentration up to critical levels (hence, a biological "traffic light").
Click the colours of the traffic light to learn about its different subparts!
