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Team:British Columbia/Safety
From 2009.igem.org
Home Team Traffic Light Sensor Lock&Key Jammer [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=British_Columbia Parts] Safety Sponsors Notebook Bibliography
Contents |
Biosafety
Biosafety issues with project, reagents or parts:
UBC iGEM's 2009 project raised no biosafety issues as no work with any pathogenic parts or organism was done. The vector recipients used are all non-pathogenic E.coli (DH5a, DB3, BW). The majority of our work was with promoters and reporters. The parts we made are modification of previously confirmed non-pathogenic molecules (Cre recombinase with LVA tag, anti-sense jammer, modified promoter). These parts alone should not pose any harm regardless of which host organism is used. Most reagents we worked with pose little to no toxic effect. Any reagents with potential for harm, for instance the DNA stains and flammable reagents, were used with appropriate care as stated and taught by our local biosafety group.
The laboratory space we used are certified for Biosafety Level 2 and all works done were well within the constraints of the certification.
Safety protocol and precautions followed:
All members of our team have taken the required Laboratory health safety course from our local biosafety group. We ensured that our members were consistent with wearing protective gears (lab coat, nitrile or latex gloves,etc). We were aware of any allergies to reagents used and appropriate precautions were taken to minimize any risk.
Local biosafety group and their view of our project:
The local biosafety group for our project is the Department of Health Safety and Environment([http://www.hse.ubc.ca/welcome.html HSE]) of UBC. Our laboratory space and equipments meets all safety requirement as per Canadian regulations and regulations of HSE. Furthermore, the laboratory space and necessary equipments have been inspected and passed.
