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Team:British Columbia/pBAD
From 2009.igem.org
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==Overview== | ==Overview== | ||
In order to make an analog biosensor, we need our traffic light to be able to respond differently to different concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the <partinfo>I13453</partinfo> pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by [[Team:British_Columbia/Bibliography|Niland et al.]] | In order to make an analog biosensor, we need our traffic light to be able to respond differently to different concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the <partinfo>I13453</partinfo> pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by [[Team:British_Columbia/Bibliography|Niland et al.]] | ||
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| + | [[Image:E_coli_Traffic_Light_Light_Mutated_Promoters|thumb||400px|A diagram of the changed nucleotide sequences of arabinose-inducible promoters. Green nucleotides of the wildtype show all sequences that were mutated to form strong and weak.]] | ||
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pbad wt ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>ATTTTTA</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... | pbad wt ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>ATTTTTA</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... | ||
pbad weak ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>TTTTTTA</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... | pbad weak ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>TTTTTTA</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... | ||
pbad strong ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>AAGATAG</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... | pbad strong ACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGC<font color=red>AAGATAG</font>TCCATAAGATTAGCGGATCCTACCTGACGCTTTTT... | ||
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Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at <partinfo>K206000</partinfo> (strong) and <partinfo>K206001</partinfo> (weak). | Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at <partinfo>K206000</partinfo> (strong) and <partinfo>K206001</partinfo> (weak). | ||
Revision as of 20:33, 20 October 2009
Overview
In order to make an analog biosensor, we need our traffic light to be able to respond differently to different concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by Niland et al.
File:E coli Traffic Light Light Mutated Promoters
A diagram of the changed nucleotide sequences of arabinose-inducible promoters. Green nucleotides of the wildtype show all sequences that were mutated to form strong and weak.
Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at (strong) and (weak).
Using these promoter sequences, we assembled XXX...
