Team:Calgary/16 July 2009

From 2009.igem.org

(Difference between revisions)
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</center>
</center>
 +
*The sequences for each primer is listed in the following table:
 +
<br>
 +
<center>
 +
{| border="1"
 +
| '''Primer''' || ‘’’Sequence’’’ ||
 +
|-
 +
| ''sig70-F1  || GCGCGCTCGAGAATAATTCTTAAAATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F2  || GCGCGCTCGAGAATAATTCTTAATATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F3 || GCGCGCTCGAGAATAATTCTTAAGATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F4 || GCGCGCTCGAGAATAATTCTTAACATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F5 || GCGCGCTCGAGAATAATTCTTTAAATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F6 ||GCGCGCTCGAGAATAATTCTTTATATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F7 || GCGCGCTCGAGAATAATTCTTTAGATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F8 || GCGCGCTCGAGAATAATTCTTTACATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F9 || GCGCGCTCGAGAATAATTCTTGAAATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F10 || GCGCGCTCGAGAATAATTCTTGATATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F11 || GCGCGCTCGAGAATAATTCTTGAGATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F12 || GCGCGCTCGAGAATAATTCTTGACATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F13 || GCGCGCTCGAGAATAATTCTTCAAATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F14 ||GCGCGCTCGAGAATAATTCTTCATATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F15 || GCGCGCTCGAGAATAATTCTTCAGATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-F16 || GCGCGCTCGAGAATAATTCTTCACATTTATGCTTCCGGCTCG
 +
|-
 +
| ''sig70-R1 || GCGGGATCCAATTGCACGTAAAATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R2 ||GCGGGATCCAATTGCACGTAATATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R3 ||GCGGGATCCAATTGCACGTAACATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R4||GCGGGATCCAATTGCACGTAAGATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R5||GCGGGATCCAATTGCACGTATAATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R6||GCGGGATCCAATTGCACGTATTATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R7 || GCGGGATCCAATTGCACGTATGATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R8 ||GCGGGATCCAATTGCACGTATCATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R9 || GCGGGATCCAATTGCACGTAGAATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R10 ||GCGGGATCCAATTGCACGTAGTATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R11 || GCGGGATCCAATTGCACGTAGGATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R12 || GCGGGATCCAATTGCACGTAGCATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R13 ||GCGGGATCCAATTGCACGTACAATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R14 || GCGGGATCCAATTGCACGTACTATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R15 ||GCGGGATCCAATTGCACGTACGATACGAGCCGGAAGCATAAA
 +
|-
 +
| ''sig70-R16 || GCGGGATCCAATTGCACGTACCATACGAGCCGGAAGCATAAA
 +
|}
 +
</center>
 +
* Stock dATP, dGTP, dCTP, dTTP from Invitrogen was diluted from 100mM to 0.5mM.
 +
* See how the primer extension PCR is done in the protocol page.
 +
* Both the varying promoters and the vector, pCS26, was digested using enzymes, XhoI and BamHI overnight. The vector was incubated at 37<sup>o</sup>C and the promoter was digested for 2 hours at 16<sup>o</sup>C and then overnight at room temperature (~21<sup>o</sup>C).
<html>
<html>
</div>
</div>

Revision as of 19:47, 29 July 2009

University of Calgary

UNIVERSITY OF CALGARY



THIS MONTH

July
MTWTFSS
    [http://2009.igem.org/Team:Calgary/1_July_2009 1] [http://2009.igem.org/Team:Calgary/2_July_2009 2] [http://2009.igem.org/Team:Calgary/3_July_2009 3] [http://2009.igem.org/Team:Calgary/4_July_2009 4] [http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6] [http://2009.igem.org/Team:Calgary/7_July_2009 7] [http://2009.igem.org/Team:Calgary/8_July_2009 8] [http://2009.igem.org/Team:Calgary/9_July_2009 9] [http://2009.igem.org/Team:Calgary/10_July_2009 10] [http://2009.igem.org/Team:Calgary/11_July_2009 11] [http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13] [http://2009.igem.org/Team:Calgary/14_July_2009 14] [http://2009.igem.org/Team:Calgary/15_July_2009 15] [http://2009.igem.org/Team:Calgary/16_July_2009 16] [http://2009.igem.org/Team:Calgary/17_July_2009 17] [http://2009.igem.org/Team:Calgary/18_July_2009 18] [http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20] [http://2009.igem.org/Team:Calgary/21_July_2009 21] [http://2009.igem.org/Team:Calgary/22_July_2009 22] [http://2009.igem.org/Team:Calgary/23_July_2009 23] [http://2009.igem.org/Team:Calgary/24_July_2009 24] [http://2009.igem.org/Team:Calgary/25_July_2009 25] [http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27] [http://2009.igem.org/Team:Calgary/28_July_2009 28] [http://2009.igem.org/Team:Calgary/29_July_2009 29] [http://2009.igem.org/Team:Calgary/30_July_2009 30] [http://2009.igem.org/Team:Calgary/31_July_2009 31]


NOTEBOOK PAGE INDEX



CALENDAR

May
MTWTFSS
        [http://2009.igem.org/Team:Calgary/1_May_2009 1] [http://2009.igem.org/Team:Calgary/2_May_2009 2] [http://2009.igem.org/Team:Calgary/3_May_2009 3]
[http://2009.igem.org/Team:Calgary/4_May_2009 4] [http://2009.igem.org/Team:Calgary/5_May_2009 5] [http://2009.igem.org/Team:Calgary/6_May_2009 6] [http://2009.igem.org/Team:Calgary/7_May_2009 7] [http://2009.igem.org/Team:Calgary/8_May_2009 8] [http://2009.igem.org/Team:Calgary/9_May_2009 9] [http://2009.igem.org/Team:Calgary/10_May_2009 10]
[http://2009.igem.org/Team:Calgary/11_May_2009 11] [http://2009.igem.org/Team:Calgary/12_May_2009 12] [http://2009.igem.org/Team:Calgary/13_May_2009 13] [http://2009.igem.org/Team:Calgary/14_May_2009 14] [http://2009.igem.org/Team:Calgary/15_May_2009 15] [http://2009.igem.org/Team:Calgary/16_May_2009 16] [http://2009.igem.org/Team:Calgary/17_May_2009 17]
[http://2009.igem.org/Team:Calgary/18_May_2009 18] [http://2009.igem.org/Team:Calgary/19_May_2009 19] [http://2009.igem.org/Team:Calgary/20_May_2009 20] [http://2009.igem.org/Team:Calgary/21_May_2009 21] [http://2009.igem.org/Team:Calgary/22_May_2009 22] [http://2009.igem.org/Team:Calgary/23_May_2009 23] [http://2009.igem.org/Team:Calgary/24_May_2009 24]
[http://2009.igem.org/Team:Calgary/25_May_2009 25] [http://2009.igem.org/Team:Calgary/26_May_2009 26] [http://2009.igem.org/Team:Calgary/27_May_2009 27] [http://2009.igem.org/Team:Calgary/28_May_2009 28] [http://2009.igem.org/Team:Calgary/29_May_2009 29] [http://2009.igem.org/Team:Calgary/30_May_2009 30] [http://2009.igem.org/Team:Calgary/31_May_2009 31]


June
MTWTFSS
[http://2009.igem.org/Team:Calgary/1_June_2009 1] [http://2009.igem.org/Team:Calgary/2_June_2009 2] [http://2009.igem.org/Team:Calgary/3_June_2009 3] [http://2009.igem.org/Team:Calgary/4_June_2009 4] [http://2009.igem.org/Team:Calgary/5_June_2009 5] [http://2009.igem.org/Team:Calgary/6_June_2009 6] [http://2009.igem.org/Team:Calgary/7_June_2009 7]
[http://2009.igem.org/Team:Calgary/8_June_2009 8] [http://2009.igem.org/Team:Calgary/9_June_2009 9] [http://2009.igem.org/Team:Calgary/10_June_2009 10] [http://2009.igem.org/Team:Calgary/11_June_2009 11] [http://2009.igem.org/Team:Calgary/12_June_2009 12] [http://2009.igem.org/Team:Calgary/13_June_2009 13] [http://2009.igem.org/Team:Calgary/14_June_2009 14]
[http://2009.igem.org/Team:Calgary/15_June_2009 15] [http://2009.igem.org/Team:Calgary/16_June_2009 16] [http://2009.igem.org/Team:Calgary/17_June_2009 17] [http://2009.igem.org/Team:Calgary/18_June_2009 18] [http://2009.igem.org/Team:Calgary/19_June_2009 19] [http://2009.igem.org/Team:Calgary/20_June_2009 20] [http://2009.igem.org/Team:Calgary/21_June_2009 21]
[http://2009.igem.org/Team:Calgary/22_June_2009 22] [http://2009.igem.org/Team:Calgary/23_June_2009 23] [http://2009.igem.org/Team:Calgary/24_June_2009 24] [http://2009.igem.org/Team:Calgary/25_June_2009 25] [http://2009.igem.org/Team:Calgary/26_June_2009 26] [http://2009.igem.org/Team:Calgary/27_June_2009 27] [http://2009.igem.org/Team:Calgary/28_June_2009 28]
[http://2009.igem.org/Team:Calgary/29_June_2009 29] [http://2009.igem.org/Team:Calgary/30_June_2009 30]


July
MTWTFSS
    [http://2009.igem.org/Team:Calgary/1_July_2009 1] [http://2009.igem.org/Team:Calgary/2_July_2009 2] [http://2009.igem.org/Team:Calgary/3_July_2009 3] [http://2009.igem.org/Team:Calgary/4_July_2009 4] [http://2009.igem.org/Team:Calgary/5_July_2009 5]
[http://2009.igem.org/Team:Calgary/6_July_2009 6] [http://2009.igem.org/Team:Calgary/7_July_2009 7] [http://2009.igem.org/Team:Calgary/8_July_2009 8] [http://2009.igem.org/Team:Calgary/9_July_2009 9] [http://2009.igem.org/Team:Calgary/10_July_2009 10] [http://2009.igem.org/Team:Calgary/11_July_2009 11] [http://2009.igem.org/Team:Calgary/12_July_2009 12]
[http://2009.igem.org/Team:Calgary/13_July_2009 13] [http://2009.igem.org/Team:Calgary/14_July_2009 14] [http://2009.igem.org/Team:Calgary/15_July_2009 15] [http://2009.igem.org/Team:Calgary/16_July_2009 16] [http://2009.igem.org/Team:Calgary/17_July_2009 17] [http://2009.igem.org/Team:Calgary/18_July_2009 18] [http://2009.igem.org/Team:Calgary/19_July_2009 19]
[http://2009.igem.org/Team:Calgary/20_July_2009 20] [http://2009.igem.org/Team:Calgary/21_July_2009 21] [http://2009.igem.org/Team:Calgary/22_July_2009 22] [http://2009.igem.org/Team:Calgary/23_July_2009 23] [http://2009.igem.org/Team:Calgary/24_July_2009 24] [http://2009.igem.org/Team:Calgary/25_July_2009 25] [http://2009.igem.org/Team:Calgary/26_July_2009 26]
[http://2009.igem.org/Team:Calgary/27_July_2009 27] [http://2009.igem.org/Team:Calgary/28_July_2009 28] [http://2009.igem.org/Team:Calgary/29_July_2009 29] [http://2009.igem.org/Team:Calgary/30_July_2009 30] [http://2009.igem.org/Team:Calgary/31_July_2009 31]


August
MTWTFSS
          [http://2009.igem.org/Team:Calgary/1_August_2009 1] [http://2009.igem.org/Team:Calgary/2_August_2009 2]
[http://2009.igem.org/Team:Calgary/3_August_2009 3] [http://2009.igem.org/Team:Calgary/4_August_2009 4] [http://2009.igem.org/Team:Calgary/5_August_2009 5] [http://2009.igem.org/Team:Calgary/6_August_2009 6] [http://2009.igem.org/Team:Calgary/7_August_2009 7] [http://2009.igem.org/Team:Calgary/8_August_2009 8] [http://2009.igem.org/Team:Calgary/9_August_2009 9]
[http://2009.igem.org/Team:Calgary/10_August_2009 10] [http://2009.igem.org/Team:Calgary/11_August_2009 11] [http://2009.igem.org/Team:Calgary/12_August_2009 12] [http://2009.igem.org/Team:Calgary/13_August_2009 13] [http://2009.igem.org/Team:Calgary/14_August_2009 14] [http://2009.igem.org/Team:Calgary/15_August_2009 15] [http://2009.igem.org/Team:Calgary/16_August_2009 16]
[http://2009.igem.org/Team:Calgary/17_August_2009 17] [http://2009.igem.org/Team:Calgary/18_August_2009 18] [http://2009.igem.org/Team:Calgary/19_August_2009 19] [http://2009.igem.org/Team:Calgary/20_August_2009 20] [http://2009.igem.org/Team:Calgary/21_August_2009 21] [http://2009.igem.org/Team:Calgary/22_August_2009 22] [http://2009.igem.org/Team:Calgary/23_August_2009 23]
[http://2009.igem.org/Team:Calgary/24_August_2009 24] [http://2009.igem.org/Team:Calgary/25_August_2009 25] [http://2009.igem.org/Team:Calgary/26_August_2009 26] [http://2009.igem.org/Team:Calgary/27_August_2009 27] [http://2009.igem.org/Team:Calgary/28_August_2009 28] [http://2009.igem.org/Team:Calgary/29_August_2009 29] [http://2009.igem.org/Team:Calgary/30_August_2009 30]
[http://2009.igem.org/Team:Calgary/31_August_2009 31]


September
MTWTFSS
  [http://2009.igem.org/Team:Calgary/1_September_2009 1] [http://2009.igem.org/Team:Calgary/2_September_2009 2] [http://2009.igem.org/Team:Calgary/3_September_2009 3] [http://2009.igem.org/Team:Calgary/4_September_2009 4] [http://2009.igem.org/Team:Calgary/5_September_2009 5] [http://2009.igem.org/Team:Calgary/6_September_2009 6]
[http://2009.igem.org/Team:Calgary/7_September_2009 7] [http://2009.igem.org/Team:Calgary/8_September_2009 8] [http://2009.igem.org/Team:Calgary/9_September_2009 9] [http://2009.igem.org/Team:Calgary/10_September_2009 10] [http://2009.igem.org/Team:Calgary/11_September_2009 11] [http://2009.igem.org/Team:Calgary/12_September_2009 12] [http://2009.igem.org/Team:Calgary/13_September_2009 13]
[http://2009.igem.org/Team:Calgary/14_September_2009 14] [http://2009.igem.org/Team:Calgary/15_September_2009 15] [http://2009.igem.org/Team:Calgary/16_September_2009 16] [http://2009.igem.org/Team:Calgary/17_September_2009 17] [http://2009.igem.org/Team:Calgary/18_September_2009 18] [http://2009.igem.org/Team:Calgary/19_September_2009 19] [http://2009.igem.org/Team:Calgary/20_September_2009 20]
[http://2009.igem.org/Team:Calgary/21_September_2009 21] [http://2009.igem.org/Team:Calgary/22_September_2009 22] [http://2009.igem.org/Team:Calgary/23_September_2009 23] [http://2009.igem.org/Team:Calgary/24_September_2009 24] [http://2009.igem.org/Team:Calgary/25_September_2009 25] [http://2009.igem.org/Team:Calgary/26_September_2009 26] [http://2009.igem.org/Team:Calgary/27_September_2009 27]
[http://2009.igem.org/Team:Calgary/28_September_2009 28] [http://2009.igem.org/Team:Calgary/29_September_2009 29] [http://2009.igem.org/Team:Calgary/30_September_2009 30]


October
MTWTFSS
      [http://2009.igem.org/Team:Calgary/1_October_2009 1] [http://2009.igem.org/Team:Calgary/2_October_2009 2] [http://2009.igem.org/Team:Calgary/3_October_2009 3] [http://2009.igem.org/Team:Calgary/4_October_2009 4]
[http://2009.igem.org/Team:Calgary/5_October_2009 5] [http://2009.igem.org/Team:Calgary/6_October_2009 6] [http://2009.igem.org/Team:Calgary/7_October_2009 7] [http://2009.igem.org/Team:Calgary/8_October_2009 8] [http://2009.igem.org/Team:Calgary/9_October_2009 9] [http://2009.igem.org/Team:Calgary/10_October_2009 10] [http://2009.igem.org/Team:Calgary/11_October_2009 11]
[http://2009.igem.org/Team:Calgary/12_October_2009 12] [http://2009.igem.org/Team:Calgary/13_October_2009 13] [http://2009.igem.org/Team:Calgary/14_October_2009 14] [http://2009.igem.org/Team:Calgary/15_October_2009 15] [http://2009.igem.org/Team:Calgary/16_October_2009 16] [http://2009.igem.org/Team:Calgary/17_October_2009 17] [http://2009.igem.org/Team:Calgary/18_October_2009 18]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/19_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/20_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/21_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/22_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/23_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/24_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/25_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Calgary/26_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/27_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/28_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/29_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/30_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Calgary/31_October_2009&preload=Team:Calgary/NotebookPreload&action=edit 31]



JULY 16, 2009


CAROL

Diluting Primers and Primer Extension Polymerase Chain Reaction (PCR)

  • To create the synthetic sigma 70 promoter library, we have synthesized 32 different primers (16 different forward primers and 16 different reverse primers) to hopefully form 256 different promoters.
  • Need to dilute primers to 80μM. The following is the concentration of the original primers that were synthesized once water was added to the primers.
Primer 260/280 260/230 Concentration [ng/μL] Reading/2.5 [ng/μL] Stock Concentration [μM] Volume stock into working [μL] Volume water into working [μL]
sig70-F1 1.98 1.73 20816 832.64 60.07 6.7 43.3
sig70-F2 1.55 1.80 44284 17713.6 1277.91 3.1 46.9
sig70-F3 1.62 1.86 4132316529.21192.463.446.6
sig70-F4 1.70 1.99 3236512946933.964.345.7
sig70-F5 1.72 2.07 3368813475.2972.144.145.9
sig70-F6 2.07 1.954541218164.81310.463.146.9
sig70-F7 1.64 1.95 3904615618.41126.763.646.4
sig70-F8 1.43 1.63 45745182981320.073.047.0
sig70-F9 1.75 2.16 236289451.2681.845.944.1
sig70-F10 1.72 2.013268213072.8943.114.245.8
sig70-F11 1.71 2.073149412597.6908.834.445.6
sig70-F12 1.59 1.82 4120216480.81188.973.446.6
sig70-F13 1.73 2.03 2922411689.6843.34.745.3
sig70-F14 1.411.58 45645182581317.23.047.0
sig70-F15 1.59 1.85 4068116272.41173.93.446.6
sig70-F16 1.57 1.82 4224116896.41219.03.346.7
sig70-R1 2.06 2.17 75163006.4216.918.431.6
sig70-R2 2.04 2.25 152556102.0440.29.140.9
sig70-R3 1.76 1.85 4250317001.21226.53.346.7
sig70-R42.012.21240129604.8692.95.844.2
sig70-R52.102.27185307412534.77.542.5
sig70-R61.982.192845211380.8821.04.945.1
sig70-R7 1.942.173297613190.4951.64.245.8
sig70-R8 2.002.202537910151.6732.45.544.5
sig70-R9 2.00 2.24 241419656.4696.65.944.1
sig70-R10 1.94 2.17 3023712094.8872.64.645.4
sig70-R11 1.922.16 3149712598.8908.94.445.6
sig70-R12 1.92 2.17 3004512018867.04.645.4
sig70-R13 2.012.18187267490.4540.47.442.6
sig70-R14 1.88 2.033611114444.41042.13.846.2
sig70-R15 1.98 2.17106794271.6308.21337
sig70-R16 1.86 2.01 3596914387.610383.946.1
  • The sequences for each primer is listed in the following table:


Primer ‘’’Sequence’’’
sig70-F1 GCGCGCTCGAGAATAATTCTTAAAATTTATGCTTCCGGCTCG
sig70-F2 GCGCGCTCGAGAATAATTCTTAATATTTATGCTTCCGGCTCG
sig70-F3 GCGCGCTCGAGAATAATTCTTAAGATTTATGCTTCCGGCTCG
sig70-F4 GCGCGCTCGAGAATAATTCTTAACATTTATGCTTCCGGCTCG
sig70-F5 GCGCGCTCGAGAATAATTCTTTAAATTTATGCTTCCGGCTCG
sig70-F6 GCGCGCTCGAGAATAATTCTTTATATTTATGCTTCCGGCTCG
sig70-F7 GCGCGCTCGAGAATAATTCTTTAGATTTATGCTTCCGGCTCG
sig70-F8 GCGCGCTCGAGAATAATTCTTTACATTTATGCTTCCGGCTCG
sig70-F9 GCGCGCTCGAGAATAATTCTTGAAATTTATGCTTCCGGCTCG
sig70-F10 GCGCGCTCGAGAATAATTCTTGATATTTATGCTTCCGGCTCG
sig70-F11 GCGCGCTCGAGAATAATTCTTGAGATTTATGCTTCCGGCTCG
sig70-F12 GCGCGCTCGAGAATAATTCTTGACATTTATGCTTCCGGCTCG
sig70-F13 GCGCGCTCGAGAATAATTCTTCAAATTTATGCTTCCGGCTCG
sig70-F14 GCGCGCTCGAGAATAATTCTTCATATTTATGCTTCCGGCTCG
sig70-F15 GCGCGCTCGAGAATAATTCTTCAGATTTATGCTTCCGGCTCG
sig70-F16 GCGCGCTCGAGAATAATTCTTCACATTTATGCTTCCGGCTCG
sig70-R1 GCGGGATCCAATTGCACGTAAAATACGAGCCGGAAGCATAAA
sig70-R2 GCGGGATCCAATTGCACGTAATATACGAGCCGGAAGCATAAA
sig70-R3 GCGGGATCCAATTGCACGTAACATACGAGCCGGAAGCATAAA
sig70-R4GCGGGATCCAATTGCACGTAAGATACGAGCCGGAAGCATAAA
sig70-R5GCGGGATCCAATTGCACGTATAATACGAGCCGGAAGCATAAA
sig70-R6GCGGGATCCAATTGCACGTATTATACGAGCCGGAAGCATAAA
sig70-R7 GCGGGATCCAATTGCACGTATGATACGAGCCGGAAGCATAAA
sig70-R8 GCGGGATCCAATTGCACGTATCATACGAGCCGGAAGCATAAA
sig70-R9 GCGGGATCCAATTGCACGTAGAATACGAGCCGGAAGCATAAA
sig70-R10 GCGGGATCCAATTGCACGTAGTATACGAGCCGGAAGCATAAA
sig70-R11 GCGGGATCCAATTGCACGTAGGATACGAGCCGGAAGCATAAA
sig70-R12 GCGGGATCCAATTGCACGTAGCATACGAGCCGGAAGCATAAA
sig70-R13 GCGGGATCCAATTGCACGTACAATACGAGCCGGAAGCATAAA
sig70-R14 GCGGGATCCAATTGCACGTACTATACGAGCCGGAAGCATAAA
sig70-R15 GCGGGATCCAATTGCACGTACGATACGAGCCGGAAGCATAAA
sig70-R16 GCGGGATCCAATTGCACGTACCATACGAGCCGGAAGCATAAA
  • Stock dATP, dGTP, dCTP, dTTP from Invitrogen was diluted from 100mM to 0.5mM.
  • See how the primer extension PCR is done in the protocol page.
  • Both the varying promoters and the vector, pCS26, was digested using enzymes, XhoI and BamHI overnight. The vector was incubated at 37oC and the promoter was digested for 2 hours at 16oC and then overnight at room temperature (~21oC).


CHINMOYEE

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EMILY

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FAHD

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IMAN

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JAMIE

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JEREMY

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KATIE

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KEVIN

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MANDY

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PATRICK

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PRIMA

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STEFAN

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VICKI

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