From 2009.igem.org

Protocols

Cloning

[http://openwetware.org/wiki/PCR PCR]

Plasmid isolation

Restriction

Gel isolation

Annealing synthetic oligo’s<h3> <h3>Ligation

Making competent cells

Transformation

Quality control

Colony PCR

Restriction analysis

List of solutions

Antibiotics

[http://openwetware.org/wiki/Ampicillin Ampicillin]

100 mg/ml Ampicillin (1000x) Stock

• 1 g of Ampicillin sodium salt in 10 mL of demiwater (or 50% EtOH)
• Add NaOH or KOH to allow the Ampicillin to dissolve
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C

[http://openwetware.org/wiki/Chloramphenicol Chloramphenicol]

35 mg/ml Chloramphenicol (1000x) Stock

• 0.35 g in 10 mL 100% EtOH
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C

[http://openwetware.org/wiki/Kanamycin Kanamycin]

50 mg/ml Kanamycin(1000x) Stock

• g in 10 mL
• Filter sterilize 0.2 μm filter and aliquot
• Store -20 °C