Team:Heidelberg/HEARTBEAT network/Results
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By using our model we were able to capture the basic behaviour of an inducible promoter (Fig.1). | By using our model we were able to capture the basic behaviour of an inducible promoter (Fig.1). | ||
+ | <!--|[[Image:HD09_NFkB_01_induc-stim.png|center|thumb|250px|'''Figure 1: ''']]--> | ||
- | Once this was accomplished, we aimed for capturing the dependency of the activity of the promoter on more heterogeneous parameters, such as affinity score, position score and presence stimulation (Figs. 2a and 2b) | + | Once this was accomplished, we aimed for capturing the dependency of the activity of the promoter on more heterogeneous parameters, such as affinity score, position score and presence stimulation (Figs. 2a and 2b). |
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|[[Image:HD09_NFkB_02aPosStim.png|none|thumb|250px|'''Figure 2a: ''']] | |[[Image:HD09_NFkB_02aPosStim.png|none|thumb|250px|'''Figure 2a: ''']] | ||
|[[Image:HD09_NFkB_02bAfStim.png|none|thumb|250x|'''Figure 2b: ''']] | |[[Image:HD09_NFkB_02bAfStim.png|none|thumb|250x|'''Figure 2b: ''']] | ||
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<!-- Fig. 1 shows the activity depending on the stimulus (yes/no) as well as on the type of promoter (inducible/constitutive). By this, high promoter activity is only observed when a promoter is inducible upon stimulation. | <!-- Fig. 1 shows the activity depending on the stimulus (yes/no) as well as on the type of promoter (inducible/constitutive). By this, high promoter activity is only observed when a promoter is inducible upon stimulation. | ||
Fig. 2(a) and (b) show how the position score (PosScore) and affinity score (AfScore) affects promoter activity given an inducible promoter. In this way it is clearly shown that promoter activity is present only if stimulating events occur. | Fig. 2(a) and (b) show how the position score (PosScore) and affinity score (AfScore) affects promoter activity given an inducible promoter. In this way it is clearly shown that promoter activity is present only if stimulating events occur. | ||
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- | [[Image:HD09_NFkB_03PosAf.png|none|thumb|320px|'''Figure 3: ''']] | + | <!--[[Image:HD09_NFkB_03PosAf.png|none|thumb|320px|'''Figure 3: ''']]--> |
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|width="250px" style="background-color:#d8d5d0" align="center"| | |width="250px" style="background-color:#d8d5d0" align="center"| | ||
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[[Image:HD09_NFkB_01_induc-stim.png|center|thumb|250px|'''Figure 1: ''']] | [[Image:HD09_NFkB_01_induc-stim.png|center|thumb|250px|'''Figure 1: ''']] | ||
<br> | <br> | ||
- | [[Image:HD09_NFkB_02aPosStim.png|none|thumb| | + | [[Image:HD09_NFkB_02aPosStim.png|none|thumb|250px|'''Figure 2a: ''']] |
+ | <br> | ||
+ | [[Image:HD09_NFkB_02bAfStim.png|none|thumb|250px|'''Figure 2b: ''']] | ||
<br> | <br> | ||
- | [[Image: | + | [[Image:HD09_NFkB_03PosAf.png|none|thumb|250px|'''Figure 3: ''']] |
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Revision as of 21:54, 19 October 2009
HEARTBEAT Fuzzy ModelingResultsModeling inducible promotersBased on prior data analysis of data derived from flow cytometry experiments using inducible synthetic NFkB responsive promoters we developed a fuzzy controller which reflects the behavior of inducible promoters. By using our model we were able to capture the basic behaviour of an inducible promoter (Fig.1). Once this was accomplished, we aimed for capturing the dependency of the activity of the promoter on more heterogeneous parameters, such as affinity score, position score and presence stimulation (Figs. 2a and 2b).
what is the model good for: importantly, our model captures the behaviour of an inducible promoter. we next wondered wheter we would be able to establish the same for an non-inducible promoter
from here: constitutives
point out: different promoter design, different experimental measuerment, as well as literature data -> final model? and then jump to final model.
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