Team:Heidelberg/Parts

From 2009.igem.org

(Difference between revisions)
(→Parts)
(→Parts)
Line 17: Line 17:
|- style="background-color:#9BDDFF;"  
|- style="background-color:#9BDDFF;"  
| style="font-weight:bold;" |pSMB_REFERENCE || Plasmid || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]
| style="font-weight:bold;" |pSMB_REFERENCE || Plasmid || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]
 +
|-
 +
|}
 +
 +
== Core promoters ==
 +
 +
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]].
 +
 +
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
 +
|- style="background-color:#99cccc;"
 +
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | JeT  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | JeT proximal-CMV core || Regulatory || [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]
 +
|-
 +
|}
 +
 +
== Promoters created by [[Team:Heidelberg/Project_Synthetic_promoters|RA-PCR]] ==
 +
 +
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
 +
 +
{| class="wikitable centered"  style="margin: 1em auto 1em auto"
 +
|- style="background-color:#99cccc;"
 +
! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Constitutive promoter; 0.4 REU  || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
 +
|- style="background-color:#9BDDFF;"
 +
| style="font-weight:bold;" | Constitutive promoter; 1 REU || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
|-
|-
|}
|}

Revision as of 13:46, 19 October 2009


Parts

Measurement Kit

Name Part type Registry link
pSMB_MEASURE: Promoter Measurement plasmid (mammalian) Plasmid backbone [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]
pSMB_REFERENCE Plasmid [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203099 Part:BBa_K203099]

Core promoters

The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.

Name Part type Registry link
JeT Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112]
JeT proximal-CMV core Regulatory [http://partsregistry.org/Part:BBa_K203113 Part:BBa_K203113]

Promoters created by RA-PCR

All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].

Name Part type Registry link
Constitutive promoter; 0.4 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203110 Part:BBa_K203110]
Constitutive promoter; 1 REU Regulatory [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203118 Part:BBa_K203118]
Retrieved from "http://2009.igem.org/Team:Heidelberg/Parts"