Team:Imperial College London/M2/genes

From 2009.igem.org

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<area coords="12,55,43,72" onmouseover="return overlib('RcsB-Pasp trancriptional activator activates transcription from associated promoter<br><b>Distance to transcription start site:</b> -45 (ranging from -51 to -39)<br><b>Evidence:</b> Gene expression analysis, Human inference based on similarity to consensus sequences, Site mutation');" onmouseout="return nd();">
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<area coords="521,41,829,72" onmouseover="return overlib(' <b>Gene:</b>  ftsZ    EG10347    b0095    ECK0096<br><b>Synonyms:</b> sfiB, sulB<br><b>Location:</b> 105,305 -> 106,456  <br> <b>Product:</b> essential cell division protein FtsZ');" onmouseout="return nd();">
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<area coords="167,41,505,72" onmouseover="return overlib(' <b>Gene:</b>  ftsA    EG10339    b0094    ECK0095<br><b>Synonyms:</b> divA<br><b>Location:</b> 103,982 -> 105,244  <br> <b>Product:</b> essential cell division protein FtsA');" onmouseout="return nd();">
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Revision as of 15:27, 11 October 2009

Contents

RcsB

Background:

RcsB is a transcription factor that forms part of a phosphorelay system. In response to membrane stress, RcsB is phosphorylated into its DNA binding form. In this state, it is able to regulate ~5% of the E.coli genome 4.


The Rcs Phosphorelay System:

In this system, RcsC encodes a membrane bound sensor kinase, while RcsB functions as the response regulator. The protein RcsA is an auxiliary activator protein that heterodimerises with RcsB to induce a mucoid phenotype. Levels of RcsA are controlled by the ATP dependent protease Lon.


RcsB upregulates the following genes:


Colanic Acid Biosynthesis:

wcaF
  • Putative colanic acid biosynthesis acetyltransferase 2
gmd

Codes for GDP-mannose 4,6-dehydratase which is a subunit of GDP-mannose 4,6-dehydratase. This enzyme is involved in colanic acid biosynthesis.

wcaD
  • Predicted colanic acid polymerase
wza
  • This codes for a lipoprotein that is required for colanic acid translocation through the outer membrane. The lipoprotein is a subunit of the capsular polysaccharide export apparatus.
fcl
  • Codes for a subunit of GDP-fucose synthase. This enzyme is involved in colanic acid biosynthesis.




General Protective:

ivy (Inhibitor of Vertebrate lysozyme)
  • Discovered in 2001 as the first bacterial lysozyme inhibitor. This Type-C lysozyme inhibitor resides in the periplasm.1
MilC (Membrane-bound lysozyme inhibitor of Type C lysozyme)
  • This is a lipoprotein that resides in the membrane. 1



OsmC (Osmotically-inducible protein C)
  • This protein provides defense against oxidative stress caused by exposure to organic hydroperoxides. 2 3


KatE
  • Codes for a subunit of heme d synthase / hydroperoxidase. This bacterial catalase protects against osmotic stress.




Global Regulators:

Sra (stationary-phase-induced ribosome-associated)

Sra is a sub-stoichiometric component of the 30S ribosomal subunit that is more abundant at stationary phase than during log phase growth.

rprA

rprA is a gene encoding a small RNA molecule that is involved in the post-transcriptional control of the alternative sigma factor, ("sigma S").


FtsA

FtsA is an essential cell division protein which colocalizes with FtsZ to the septal ring structure.

FtsZ
  • Essential cell division protein.
yfbR
  • dCMP phosphohydrolase


Membrane Proteins:

bdm (biofilm-dependent modulation protein)
OsmB
  • OsmB encodes an outer membrane lipoprotein.
OsmY

Hyperosmotically inducible periplasmic protein

PhnQ
ycfJ
  • Uncharacterized putative inner membrane protein.
ygaC
spy
ymgD
  • Putative periplasmic protein.
ypeC
  • Unknown function


ymgG
YbaY

Predicted outer membrane lipoprotein




References

1) [http://www.ncbi.nlm.nih.gov/pubmed/19136591 The Rcs two-component system regulates expression of lysozyme inhibitors and is induced by exposure to lysozyme]

2) [http://www.ncbi.nlm.nih.gov/pubmed/19346340 Gene Expression Induced in Escherichia coli O157:H7 upon Exposure to Model Apple Juice]

3) [http://www.ncbi.nlm.nih.gov/pubmed/14627744 Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC.]

4) [http://www.ncbi.nlm.nih.gov/pubmed/16153174?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum The Rcs phosphorelay: a complex signal transduction system.]



























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