Team:Imperial College London/Notebook

From 2009.igem.org

(Difference between revisions)
 
(38 intermediate revisions not shown)
Line 1: Line 1:
{{Imperial/09/TemplateTop}}
{{Imperial/09/TemplateTop}}
 +
{{Imperial/09/Tabs/Main/Wetlab/Protocols}}
=Notebook=
=Notebook=
Line 6: Line 7:
Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments. <br>
Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments. <br>
<br>
<br>
-
{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09}}
+
{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=09 | left}}{{#Calendar: title=Imperial_College_London/Notebook |year=2009 | month=10 | left}}
{{Imperial/09/Division}}
{{Imperial/09/Division}}
-
=Progress=
 
-
This table shows our progress with each part:
+
=BioBrick Progress=
 +
 
 +
The table below shows the progress/status of each BioBrick part/construct:
{| width="100%" style="background-color:transparent;"
{| width="100%" style="background-color:transparent;"
|
|
-
{| border="2" cellpadding="2" style="text-align:center" align="left" width="30%"
+
{| border="2" cellpadding="2" style="text-align:left" align="left" width="30%"
! Key
! Key
|- style=background:LimeGreen
|- style=background:LimeGreen
Line 22: Line 24:
| Sequenced
| Sequenced
|- style=background:MediumSlateBlue
|- style=background:MediumSlateBlue
-
| Midiprepped
+
| Midi-prepped
|- style=background:GreenYellow
|- style=background:GreenYellow
| Fully verified
| Fully verified
|- style=background:MediumVioletRed
|- style=background:MediumVioletRed
-
| Partially Verified
+
| Partially verified
-
|- style=background:LightSeaGreen
+
|- style=background:Orchid
| Transformed
| Transformed
|- style=background:DarkOrange
|- style=background:DarkOrange
Line 34: Line 36:
| SLIC
| SLIC
|- style=background:Red
|- style=background:Red
-
| Cloning difficulties
+
| Site-directed mutagenesis
 +
|- style=background:YellowGreen
 +
| Awaiting Mr.Gene
|}
|}
|}
|}
Line 40: Line 44:
{| border="2" cellpadding="2" style="text-align:left" align="left" width="100%"
{| border="2" cellpadding="2" style="text-align:left" align="left" width="100%"
!Part Number !! Part Name !! Status  
!Part Number !! Part Name !! Status  
-
|- style=background:LimeGreen
 
-
|  || || Submitted
 
-
|- style=background:GreenYellow
 
-
|  || || Fully verified
 
-
|- style=background:DeepSkyBlue
 
-
|  || || Sequenced
 
-
|- style=background:MediumVioletRed
 
-
|  || || Partially Verified
 
|- style=background:Red
|- style=background:Red
-
|  || || Cloning difficulties
+
|  BBa_K200000 ||RcsB|| Sequenced from mini-prep/SDM on midi-prep
-
|- style=background:Yellow
+
|- style=background:MediumVioletRed
-
|  || || SLIC
+
|  BBa_K200001 ||Dam methylase|| Mini-prepped
 +
|- style=background:MediumVioletRed
 +
| BBa_K200002  ||ygiV (B3023)|| Mini-prepped
 +
|- style=background:MediumVioletRed
 +
| BBa_K200003  ||Waal Ligase|| Mini-prepped
|- style=background:MediumSlateBlue
|- style=background:MediumSlateBlue
-
|  || || Midiprepped
+
| BBa_K200005 ||OtsA|| Sequenced from midi-prep
 +
|- style=background:MediumSlateBlue
 +
| BBa_K200006  ||OtsB|| Sequenced from midi-prep
 +
|- style=background:Orchid
 +
| BBa_K200007  ||Cellulase|| Transformed
 +
|- style=background:Red
 +
| BBa_K200008  ||Phenylalanine hydroxylase|| Site-directed mutagenesis/mini-preps
 +
|- style=background:YellowGreen
 +
| BBa_K200009  ||DpnII restriction enzyme|| Awaiting Mr. Gene
 +
|- style=background:Orchid
 +
| BBa_K200009  ||TaqI restriction enzyme (RBS+TaqI+TT)|| Transformed
 +
|- style=background:YellowGreen
 +
| BBa_K200011  ||Lambda cI repressor|| Awaiting Mr. Gene
 +
|- style=background:DeepSkyBlue
 +
| BBa_K200012  ||Lambda promoter|| Sequenced from mini-prep
 +
|- style=background:DeepSkyBlue
 +
| BBa_  ||pLacI|| Sequenced from mini-prep
 +
|- style=background:DeepSkyBlue
 +
| BBa_  ||pCstA|| Sequenced from mini-prep
 +
|- style=background:Orchid
 +
| BBa_  ||pCstA+RBS+GFP+TT|| Transformed
 +
|- style=background:Orchid
 +
| BBa_  ||pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT|| Transformed
|- style=background:DarkOrange
|- style=background:DarkOrange
-
|  || || Ligation pending/Failed
+
| BBa_ ||RBS+OtsB|| Mini-prepped
-
|- style=background:LightSeaGreen
+
|- style=background:MediumVioletRed
-
|  || || Transformed
+
| BBa_ ||pCstA+RBS|| Mini-prepped
 +
|- style=background:MediumVioletRed
 +
| BBa_  ||LacI+RBS|| Mini-prepped
|}
|}

Latest revision as of 21:02, 13 October 2009



Notebook

Here is our daily notebook.

Please update all the experiments that you have performed, and any considerations along the way so that everyone can see the results and others can duplicate your experiments.

September
MTWTFSS
  [http://2009.igem.org/Imperial_College_London/Notebook/1_September_2009 1] [http://2009.igem.org/Imperial_College_London/Notebook/2_September_2009 2] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/3_September_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/4_September_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/6_September_2009&action=edit 6]
[http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/7_September_2009&action=edit 7] [http://2009.igem.org/Imperial_College_London/Notebook/8_September_2009 8] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/9_September_2009&action=edit 9] [http://2009.igem.org/Imperial_College_London/Notebook/10_September_2009 10] [http://2009.igem.org/Imperial_College_London/Notebook/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/13_September_2009&action=edit 13]
[http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/14_September_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/15_September_2009&action=edit 15] [http://2009.igem.org/Imperial_College_London/Notebook/16_September_2009 16] [http://2009.igem.org/Imperial_College_London/Notebook/17_September_2009 17] [http://2009.igem.org/Imperial_College_London/Notebook/18_September_2009 18] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/20_September_2009&action=edit 20]
[http://2009.igem.org/Imperial_College_London/Notebook/21_September_2009 21] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/22_September_2009&action=edit 22] [http://2009.igem.org/Imperial_College_London/Notebook/23_September_2009 23] [http://2009.igem.org/Imperial_College_London/Notebook/24_September_2009 24] [http://2009.igem.org/Imperial_College_London/Notebook/25_September_2009 25] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/27_September_2009&action=edit 27]
[http://2009.igem.org/Imperial_College_London/Notebook/28_September_2009 28] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/29_September_2009&action=edit 29] [http://2009.igem.org/Imperial_College_London/Notebook/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Imperial_College_London/Notebook/1_October_2009 1] [http://2009.igem.org/Imperial_College_London/Notebook/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/4_October_2009&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/5_October_2009&action=edit 5] [http://2009.igem.org/Imperial_College_London/Notebook/6_October_2009 6] [http://2009.igem.org/Imperial_College_London/Notebook/7_October_2009 7] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/8_October_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/9_October_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/11_October_2009&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/12_October_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/13_October_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/14_October_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/15_October_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/16_October_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/17_October_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/18_October_2009&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/19_October_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/20_October_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/21_October_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Imperial_College_London/Notebook/31_October_2009&action=edit 31]


BioBrick Progress

The table below shows the progress/status of each BioBrick part/construct:

Key
Submitted
Sequenced
Midi-prepped
Fully verified
Partially verified
Transformed
Ligation pending/Failed
SLIC
Site-directed mutagenesis
Awaiting Mr.Gene


Part Number Part Name Status
BBa_K200000 RcsB Sequenced from mini-prep/SDM on midi-prep
BBa_K200001 Dam methylase Mini-prepped
BBa_K200002 ygiV (B3023) Mini-prepped
BBa_K200003 Waal Ligase Mini-prepped
BBa_K200005 OtsA Sequenced from midi-prep
BBa_K200006 OtsB Sequenced from midi-prep
BBa_K200007 Cellulase Transformed
BBa_K200008 Phenylalanine hydroxylase Site-directed mutagenesis/mini-preps
BBa_K200009 DpnII restriction enzyme Awaiting Mr. Gene
BBa_K200009 TaqI restriction enzyme (RBS+TaqI+TT) Transformed
BBa_K200011 Lambda cI repressor Awaiting Mr. Gene
BBa_K200012 Lambda promoter Sequenced from mini-prep
BBa_ pLacI Sequenced from mini-prep
BBa_ pCstA Sequenced from mini-prep
BBa_ pCstA+RBS+GFP+TT Transformed
BBa_ pCstA+RBS+GFP+TT+pLacI+RBS+RFP+TT Transformed
BBa_ RBS+OtsB Mini-prepped
BBa_ pCstA+RBS Mini-prepped
BBa_ LacI+RBS Mini-prepped


Mr. Gene   Geneart   Clontech   Giant Microbes

Retrieved from "http://2009.igem.org/Team:Imperial_College_London/Notebook"