Team:Imperial College London/Stomach

From 2009.igem.org

(Difference between revisions)
(Peptide cutter)
(Undo revision 81771 by Droche (Talk))
Line 39: Line 39:
<html>
<html>
<body>
<body>
-
<!-- ********************************************************************************************** -->
 
-
<!-- START OF HEADER information on top of the page -->
 
<div id='sib_container'>
<div id='sib_container'>
   <div id = 'sib_body'>     
   <div id = 'sib_body'>     
   <b>PeptideCutter</b>
   <b>PeptideCutter</b>
-
   <form method=POST action="http://www.expasy.ch/cgi-bin/peptidecutter/peptidecutter.pl" target="_blank">  
+
   <form method=POST action="http://www.expasy.ch/cgi-bin/peptidecutter/peptidecutter.pl" target="_blank">
 +
  <textarea name="protein" rows=12 cols=50></textarea>
 +
   
   <input type="submit" value="Perform"> the cleavage of the protein.  
   <input type="submit" value="Perform"> the cleavage of the protein.  
   <input type="reset" value="Reset"> the fields.<P>
   <input type="reset" value="Reset"> the fields.<P>

Revision as of 10:56, 2 October 2009

Contents

Human Digestive Proteases:

The stomach serves to break large proteins into peptides. These peptides are then broken down into amino acids once they enter the duodenum.


  • Pepsin = stomach
  • Trypsin = duodenum
  • Chymotrypsin
  • Carboxypeptidase



Stomach

The stomach is the first point at which polypeptides are broken down. The low pH of the stomach causes enzymes to denature, opening them up to attack from proteases such as pepsin.

Pepsin

  • Released by chief cells in the stomach.
  • Expressed as a zymogen pepsinogen.
  • Pepsinogen is converted to pepsin by HCl which is released by parietal cells of the stomach.
  • Cleaves at the N-terminus after aromatic amino acids such as phenylalanine, tryptophan, and tyrosine.
  • Optimum pH of 1.5 to 2. Pepsin denatures when the pH is more than 5.0.



Peptide cutter

PeptideCutter
the cleavage of the protein. the fields.


references /documentation

Mr. Gene   Geneart   Clontech   Giant Microbes