Team:Imperial College London/Stomach

From 2009.igem.org

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[[Image:Digestivesystem.jpg]]
The stomach serves to break large proteins into peptides. These peptides are then broken down into amino acids once they enter the duodenum.
The stomach serves to break large proteins into peptides. These peptides are then broken down into amino acids once they enter the duodenum.

Revision as of 12:38, 2 October 2009

Contents

Human Digestive Proteases:

Digestivesystem.jpg

The stomach serves to break large proteins into peptides. These peptides are then broken down into amino acids once they enter the duodenum.


  • Pepsin = stomach
  • Trypsin = duodenum
  • Chymotrypsin
  • Carboxypeptidase



Stomach

The stomach is the first point at which polypeptides are broken down. The low pH of the stomach causes enzymes to denature, opening them up to attack from proteases such as pepsin.

Pepsin

Pepsin is a powerful protease that cleaves at the N-terminus after aromatic amino acids such as phenylalanine, tryptophan, and tyrosine. The optimum pH of pepsin is from 1.5 to 2, at a pH of above 5 denaturation occurs. This means that pepsin is only functional in the acidic environment of the stomach.

One of the enzymes that we are showcasing is phenylalanine hydroxylase (PAH) (as a treatment for PKU). If this enzyme was simply swallowed in an unencapsulated form it would be cleaved in 146 places by pepsin in the stomach.

Click on the perform button to see a simulated digestion of PAH by pepsin. Alternativly, paste in your own sequence to see if it would be a good candidate for encapsulation.


references /documentation


Duodenum

Duodenum.png

Endopeptidases (trypsin, chymotryopsin, elastase) Exopeptidases (carboxypeptidases A & B) Enteropeptidase



references /documentation





Trypsin

  • Trypsin is found in the duodenum and serves to hydrolyse peptides into amino acids.
  • Trypsin has an optimal operating pH of ~8 and an optimum temperature of 37°C.
  • Trypsin breaks down the milk protein casein. For this reason, milk protein would be a good protein to use for secondary encapsulation.

Chymotryopsin

Elastase

Enterokinase

Carboxypeptidases A & B


Small Intestine = Aminopeptidase N Dipeptidyl aminopeptidase IV Aminopeptidase P Carboxypeptidase P Angotensin-converting enzyme Glutamyl aminopeptidase

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