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Team:Imperial College London/Temporal Control
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| - | =[[Image:II09_Temporal_control.png|50px]]<font face='Calibri' size='5'><b> | + | =[[Image:II09_Temporal_control.png|50px]]<font face='Calibri' size='5'><b>Module Integration</b></font>= |
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| - | <img src="https://static.igem.org/mediawiki/2009/ | + | <img src="https://static.igem.org/mediawiki/2009/2/29/Ii09_tcg1.PNG" alt="" title="Testing Click to expand" width="100%"/> |
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| + | <font face='Calibri' size='4'><b>Engineering Approach to Module Integration by <u>Temporal Control</u></b></font> | ||
| + | This <b>temporal control</b> platform showcases our engineering approach in the E.ncapsulator project. It allows us to integrate all our modules in a simple and elegant way. | ||
| + | We’ve made our entire system <b>modular</b>, where we trigger the succession of events using this control system. Here, we have tackled a drug delivery problem, but this "Black box" approach can be applied to any other system. This is our <b>novel engineering approach</b>, which is extremely <b>reusable</b> in synthetic biology. | ||
| - | === | + | [[Image:II09_BlackBox3.png|400px|center]]<br> |
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| + | <!--<html> | ||
| + | <div class="highslide-gallery" align="center"> | ||
| + | <a href="https://static.igem.org/mediawiki/2009/3/3c/Ii09_tempotxt.PNG" class="highslide" onclick="return hs.expand(this)"> | ||
| + | <img src="https://static.igem.org/mediawiki/2009/3/3c/Ii09_tempotxt.PNG" alt="" title="Testing Click to expand" width="100%"/> | ||
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| + | Temporal Control | ||
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| - | ====Testing Construct==== | + | <!--====Testing Construct==== |
This testing construct was used to test the inducible promoters using flourescent proteins as output reporters.<br> | This testing construct was used to test the inducible promoters using flourescent proteins as output reporters.<br> | ||
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| + | ===Timeline of Temporal Control=== | ||
This timeline shows the sequence of occurrence of these events: | This timeline shows the sequence of occurrence of these events: | ||
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<a href="https://static.igem.org/mediawiki/2009/1/15/II09_Timeline.png" class="highslide" onclick="return hs.expand(this)"> | <a href="https://static.igem.org/mediawiki/2009/1/15/II09_Timeline.png" class="highslide" onclick="return hs.expand(this)"> | ||
<img src="https://static.igem.org/mediawiki/2009/1/15/II09_Timeline.png" alt="" title="Module 3 is the destruction of the genetic material." width="95%"/> | <img src="https://static.igem.org/mediawiki/2009/1/15/II09_Timeline.png" alt="" title="Module 3 is the destruction of the genetic material." width="95%"/> | ||
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| + | <a href="https://static.igem.org/mediawiki/2009/3/3d/Ii09_tcg4.PNG" class="highslide" onclick="return hs.expand(this)"> | ||
| + | <img src="https://static.igem.org/mediawiki/2009/3/3d/Ii09_tcg4.PNG" alt="" title="Module 3 is the destruction of the genetic material." width="95%"/> | ||
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| + | <img src="https://static.igem.org/mediawiki/2009/7/7f/Ii09_tcg7.PNG" alt="" title="Module 3 is the destruction of the genetic material." width="95%"/> | ||
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| + | <a href="https://static.igem.org/mediawiki/2009/8/80/Ii09_tcg8.PNG" class="highslide" onclick="return hs.expand(this)"> | ||
| + | <img src="https://static.igem.org/mediawiki/2009/8/80/Ii09_tcg8.PNG" alt="" title="Module 3 is the destruction of the genetic material." width="95%"/> | ||
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Graph#Explanation_of_Timeline | <html><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Graph#Explanation_of_Timeline | ||
| - | "><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/ | + | "><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09Learnmore.png"></a></html> <b>About the timeline, its explainations, and to view our testing construct.</b> |
<!--In our project there are 3 forms of temporal control that have been implemented. | <!--In our project there are 3 forms of temporal control that have been implemented. | ||
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| - | ===Our Results=== | + | <!--===Our Results=== |
<u>Wetlab</u> | <u>Wetlab</u> | ||
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results#Autoinduction | <html><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results#Autoinduction | ||
| - | "><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/ | + | "><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09Learnmore.png"></a></html> About the wetlab results! |
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction | <html><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction | ||
| - | "><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/ | + | "><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09Learnmore.png"></a></html> about the models and simulations! |
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| - | === | + | ==Conclusion== |
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| - | + | Module integration has allowed us to link all our modules together, to create a working network. | |
| - | + | It also allows us to showcase the advantages of <b>synthetic biology</b> as the <b>marriage of engineering with biology</b>. By integrating the engineering principles of reusability and simplicity of system, we have developed this platform to program biologcal systems. | |
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| + | <br> | ||
| + | <center> | ||
| + | ===Project Tour=== | ||
| + | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M3"><img width=150px src="http://i691.photobucket.com/albums/vv271/dk806/Module3L.jpg"></a> | ||
| + | <a href="https://2009.igem.org/Team:Imperial_College_London/Genetic_Circuit"><img width=150px src="http://i691.photobucket.com/albums/vv271/dk806/GeneticCircuitsR.jpg"></a> | ||
| + | </html> | ||
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===Temporal Control Contents=== | ===Temporal Control Contents=== | ||
| - | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/ | + | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_chemicalinduction.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Autoinduction"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage1.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Thermoinduction"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Thermoinduction1.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results#Temporal_Control"><img style="vertical-align:bottom;"width="20%"src="http://i691.photobucket.com/albums/vv271/dk806/II09_Wetlabmainimage9.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage6.png"></a></center></html> |
<html><table border="0" style="background-color:transparent;" width="100%"> | <html><table border="0" style="background-color:transparent;" width="100%"> | ||
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<td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Autoinduction"><b>Autoinduction</b></a></center></td> | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Autoinduction"><b>Autoinduction</b></a></center></td> | ||
<td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Thermoinduction"><b>Thermoinduction</b></a></center></td> | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Thermoinduction"><b>Thermoinduction</b></a></center></td> | ||
| - | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/ | + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results#Temporal_Control"><b>Wet Lab</b></a></center></td> |
| - | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/ | + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab/Autoinduction"><b>Modelling</b></a></center></td> |
<tr><td width="1%"></td> | <tr><td width="1%"></td> | ||
Latest revision as of 01:00, 21 October 2009
Contents |
Module Integration
Engineering Approach to Module Integration by Temporal Control
This temporal control platform showcases our engineering approach in the E.ncapsulator project. It allows us to integrate all our modules in a simple and elegant way.
We’ve made our entire system modular, where we trigger the succession of events using this control system. Here, we have tackled a drug delivery problem, but this "Black box" approach can be applied to any other system. This is our novel engineering approach, which is extremely reusable in synthetic biology.
Timeline of Temporal Control
This timeline shows the sequence of occurrence of these events:
About the timeline, its explainations, and to view our testing construct.
Conclusion
Module integration has allowed us to link all our modules together, to create a working network.
It also allows us to showcase the advantages of synthetic biology as the marriage of engineering with biology. By integrating the engineering principles of reusability and simplicity of system, we have developed this platform to program biologcal systems.
Project Tour
Temporal Control Contents
