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Team:Imperial College London/Temporal Control
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=Introduction= | =Introduction= | ||
In the <b><i>E.ncapsulator</i></b>, temporal control is an integral part of the design. The 3 modules can only perform their function in this system if they have a temporal mechanism triggering them in a sequential manner. We have achieved this using 3 types of control: | In the <b><i>E.ncapsulator</i></b>, temporal control is an integral part of the design. The 3 modules can only perform their function in this system if they have a temporal mechanism triggering them in a sequential manner. We have achieved this using 3 types of control: | ||
| - | *<b>Chemical induction:</b> | + | *<b>Chemical induction:</b> Starts protein drug production ([https://2009.igem.org/Team:Imperial_College_London/M1 Module1]). |
| - | *<b>Autoinduction</b>: | + | *<b>Autoinduction</b>: Starts Encapsulation ([https://2009.igem.org/Team:Imperial_College_London/M2 Module 2]). |
| - | *<b>Thermoinduction</b>: | + | *<b>Thermoinduction</b>: Starts Genomic Deletion. ([https://2009.igem.org/Team:Imperial_College_London/M3 Module 3]). |
== Chemical induction == | == Chemical induction == | ||
Revision as of 21:02, 16 September 2009
Contents |
Introduction
In the E.ncapsulator, temporal control is an integral part of the design. The 3 modules can only perform their function in this system if they have a temporal mechanism triggering them in a sequential manner. We have achieved this using 3 types of control:
- Chemical induction: Starts protein drug production (Module1).
- Autoinduction: Starts Encapsulation (Module 2).
- Thermoinduction: Starts Genomic Deletion. (Module 3).
Chemical induction
From the Module 1 genetic circuit, in the absence of IPTG in the system, the LacI repressor inhibits production of the protein of interest. When IPTG is added in we start the production of the drug of interest:
[For more details of the system, click here- (bits of Charles' literature review), includes info on lac operon and how we have modified it]
Autoinduction
[Diauxic growth and autoinduction media]
[click for more details... link to models too]
Thermoinduction
c
